We narrowed to 9,264 results for: pho

1. sgRNA3_NANOG

   Plasmid

   #64155

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)

   Plasmid

   #15384

   Depositor

   Linda Boxer

   Article

   Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Bacterial and Mammalian

   Mutation

   Bcl-2 full promoter with IgH full enhancer MHS123…

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

4. FUG-PtenN276S

   Plasmid

   #140737

   Purpose

   Lentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   PTEN – phosphatase and tensin homolog (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N276S

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF4Ag NanoLuc (ATG-42)

   Plasmid

   #137777

   Purpose

   Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

   Depositor

   Lance Encell

   Article

   Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

   Insert

   NanoLuc (Nluc)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NanoLuc contains 16 amino acid changes compared t…

   Promoter

   T7 and CMV

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJM656

   Plasmid

   #53086

   Purpose

   A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

   Depositor

   Ding Xue

   Article

   Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

   Insert

   Lactaherin (Mfge8 Mouse)
   

   Use

   Tags

   GFP (w/ introns) and signal sequence

   Expression

   Worm

   Mutation

   deleted native signal sequence, deleted C-termina…

   Promoter

   hsp-16.41

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mCherry-PA-Rac1

   Plasmid

   #22027

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-PA-Rac1

    Plasmid

    #22024

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-ALK3 wt

    Plasmid

    #80873

    Purpose

    mammalian expression of ALK3 WT

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK3 (BMPR1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-v2 RsTAL At4CL

    Plasmid

    #126524

    Purpose

    expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

    Depositor

    Andrew Woolley

    Article

    Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

    Inserts

    tyrosine ammonia lyase
    p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0144

    Plasmid

    #141536

    Purpose

    Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PHOX2A (PHOX2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-C2m2-SNAP

    Plasmid

    #208773

    Purpose

    To express C2m2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-SNAP (Mfge8 Mouse)
    

    Use

    Tags

    SNAP-tag

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-PA-Rac1

    Plasmid

    #22007

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-FLAG-HA-CAD S1859A

    Plasmid

    #46239

    Purpose

    FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1859 changed to Alanine (S1859A)

    Promoter

    cmv

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB1N-LZ-LOV2(wt)

    Plasmid

    #107614

    Purpose

    N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    CMV

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-PPO-Venus

    Plasmid

    #139502

    Purpose

    Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Use

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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