We narrowed to 18,060 results for: Gateway

1. pDONR221_SLC6A18

   Plasmid

   #132219

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A18 (SLC6A18 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR221_SLC25A14

   Plasmid

   #132050

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A14 (SLC25A14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pDONR221_SLC25A29

   Plasmid

   #132028

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A29 (SLC25A29 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDONR221_SLC25A35

   Plasmid

   #132017

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A35 (SLC25A35 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR221_SLC25A2

   Plasmid

   #132015

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A2 (SLC25A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDONR221_SLC25A41

   Plasmid

   #132006

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A41 (SLC25A41 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR221_RHCG

   Plasmid

   #131895

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   RHCG (RHCG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLgw NKX2-5-V5-EcoDam

   Plasmid

   #98597

   Purpose

   Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLgw EcoDam-V5-NKX2-5Y191C

   Plasmid

   #98598

   Purpose

   Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Y191C point mutant

   Promoter

   Heat Shock Minimal Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid

    #98599

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KHBD00026

    Plasmid

    #34188

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8333 (E(spl)mgamma-HLH Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST51-LRRK2-G2019S

    Plasmid

    #29401

    Depositor

    Mark Cookson

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Gly2019Ser

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST51-LRRK2-R1398Q

    Plasmid

    #29398

    Depositor

    Mark Cookson

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Arg1398Gln

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE mCherry-Rab11A

    Plasmid

    #228035

    Purpose

    Mammalian lentiviral expression of mCherry-labeled Rab11.

    Depositor

    Wade Harper

    Insert

    Rab11A (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LZRS-Rfa

    Plasmid

    #31601

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IDG_CACNG8_OE_1

    Plasmid

    #161698

    Purpose

    Overexpresses CACNG8

    Depositor

    Mike McManus

    Insert

    CACNG8 (CACNG8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Phage-PGK-GFP-T(WT)

    Plasmid

    #181736

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid

    #169389

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP16
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCLX-R4-DEST-R2

    Plasmid

    #45956

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Methods Mol Biol. 2011;737:183-209. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLEX307-TMPRSS4-puro

    Plasmid

    #158472

    Purpose

    Constitutive lentiviral expression of human TMPRSS4.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS4 (TMPRSS4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG426-GAL-UBQLN2

    Plasmid

    #181708

    Purpose

    Galactose inducible expression of UBQLN2

    Depositor

    Alejandro Chavez

    Insert

    UBQLN2 (UBQLN2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCWXPG-UBI-Glis3M412

    Plasmid

    #114301

    Purpose

    Ubiquitous expression of a constitutively active form of the Glis3 transcription factor

    Depositor

    Patrick Salmon

    Insert

    Glis3 (Glis3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N-terminal deletion up to Methionine 412

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid

    #78332

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pENTR223 TMPRSS2, closed

    Plasmid

    #149721

    Purpose

    for Gateway cloning of TMPRSS2 into destination vector

    Depositor

    Anne-Claude Gingras

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    wt, without start codon

    Promoter

    Available since

    June 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pPtGG-1

    Plasmid

    #89990

    Purpose

    pBR322-based entry vector for Gateway cloning

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid

    #169384

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP10
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. IDG_CACNG7_OE_1

    Plasmid

    #161651

    Purpose

    Overexpresses CACNG7

    Depositor

    Mike McManus

    Insert

    CACNG7 (CACNG7 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid

    #169391

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF3b
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAM-35s-CBL2-YFPc-1

    Plasmid

    #102394

    Purpose

    split YFP. Plant expression of CBL2-YFPc-1

    Depositor

    Silke Robatzek

    Insert

    CBL2 (CBL2 Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti4/V5-DEST-P85(KS459del)

    Plasmid

    #40222

    Depositor

    Lynda Chin

    Insert

    P85(KS459delN) (PIK3R1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    amino acids 459-460 replaced with asparagine

    Promoter

    CMV

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pENTR-HLA-B15-His

    Plasmid

    #104837

    Purpose

    HLA-B150101 cloned as a His tag

    Depositor

    Sébastien Walchli

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Entry vector for gateway system

    Tags

    his

    Expression

    Mutation

    2 differences compared to original B15: L2->R …

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid

    #78334

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pDEST51-LRRK2-I1371V

    Plasmid

    #29399

    Depositor

    Mark Cookson

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Ile1371Val

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

37. IDG_CACNG6_OE_1

    Plasmid

    #161696

    Purpose

    Overexpresses CACNG6

    Depositor

    Mike McManus

    Insert

    CACNG6 (CACNG6 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDEST51-LRRK2-T1343G

    Plasmid

    #29400

    Depositor

    Mark Cookson

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Thr1343Gly

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pLEX307-TMPRSS4-blast

    Plasmid

    #158473

    Purpose

    Constitutive lentiviral expression of human TMPRSS4.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS4 (TMPRSS4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti4/V5-DEST-P85(R574fs)

    Plasmid

    #40227

    Depositor

    Lynda Chin

    Insert

    P85(R574fs) (PIK3R1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Introduced frame shift mutation at amino acid 574

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only