We narrowed to 5,882 results for: arl

1. pRK5-EGFP-Tau E14

   Plasmid

   #46907

   Purpose

   expresses EGFP tagged Tau E14 in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau E14 (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T1 human PP2A Aα full length

   Plasmid

   #132634

   Purpose

   expresses human PP2A Aα full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

   Insert

   PP2A Aα (PPP2R1A Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-EGFP-Tau E14 P301L

   Plasmid

   #46909

   Purpose

   expresses EGFP tagged Tau E14 P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau E14 P301L (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L AND all 14 S/P or T/P amino acid residues (…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5-EGFP-Tau AP P301L

   Plasmid

   #46906

   Purpose

   expresses EGFP tagged Tau AP P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP P301L (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L AND all 14 S/P or T/P amino acid residues (…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX209-neo-WRN K577M

   Plasmid

   #125790

   Purpose

   constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   myc-WRN K577M (WRN Human)
   

   Tags

   5x myc

   Expression

   Mammalian

   Mutation

   K577M (helicase-dead)

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET_His6-SUMO-TEV-TtCsm6

   Plasmid

   #172486

   Purpose

   Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
   

   Tags

   His-SUMO-TEV

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX209-neo-WRN E84A

   Plasmid

   #125789

   Purpose

   constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   myc-WRN E84A (WRN Human)
   

   Tags

   5x myc

   Expression

   Mammalian

   Mutation

   E84A (exonuclease-dead)

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX209-neo-WRN E84A K577M

   Plasmid

   #125791

   Purpose

   constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   myc-WRN E84A/K577M (WRN Human)
   

   Tags

   5x myc

   Expression

   Mammalian

   Mutation

   E84A/K577M (exonuclease/helicase-dead)

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-Flag-SPRTN-wt

   Plasmid

   #110214

   Purpose

   Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Expression

   Mammalian

   Mutation

   P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_hSyn-PdCO-EGFP-WPRE

    Plasmid

    #198513

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Capping Protein

    Plasmid

    #89950

    Purpose

    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.

    Depositor

    Antoine Jégou, Guillaume Romet-Lemonne

    Article

    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.

    Inserts

    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    V222I

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

    Plasmid

    #223959

    Purpose

    Dual fluorescent reporter for histone H2B and for APC/C activity

    Depositor

    Hee Won Yang

    Article

    Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

    Insert

    H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet-O-FUW-EGR1-P2A-mCherry

    Plasmid

    #203856

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR1 (EGR1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T1 human PP2A B56α full length

    Plasmid

    #132635

    Purpose

    expresses human PP2A B56α full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A B56α
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-KrasG12D/sgKras/Cre

    Plasmid

    #99851

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

    Plasmid

    #129180

    Purpose

    Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Use

    AAV

    Tags

    Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

    Promoter

    CAG

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-KrasG12C/sgKras/Cre

    Plasmid

    #99850

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_N-DYK_MTHFD1-NLS

    Plasmid

    #133023

    Purpose

    NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-KrasG12V/sgKras/Cre

    Plasmid

    #99854

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_BRD051-sgMLH1-1

    Plasmid

    #125776

    Purpose

    constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgMLH1-1 (MLH1 Human)
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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