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Showing: 10401 - 10450 of 32228 results

  2. pJo55

    Plasmid
    #159623
    Purpose
    An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.
    Depositor
    Vasiliy Taranov
    Article
    Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    159623-plasmid-map-sequence-id-329054

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  4. pBAD-MtrxRFP2

    Plasmid
    #172323
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172323-plasmid-map-sequence-id-431098

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  15. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168258-plasmid-map-sequence-id-332729

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  16. pMRBad-C-wtBlc

    Plasmid
    #168257
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    C-fragment of wtBlc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168257-plasmid-map-sequence-id-332728

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  17. pMRBad-Z-C-wtBlc

    Plasmid
    #168256
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    Leucine Zipper + C-fragment of wtBlc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168256-plasmid-map-sequence-id-332726

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  18. Tol2-GgVCL/WT

    Plasmid
    #162787
    Purpose
    CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Use
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    beta actin
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162787-plasmid-map-sequence-id-327628

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  19. PB-TAC-Cas9

    Plasmid
    #120352
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120352-plasmid-map-sequence-id-237238

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  20. PB-TAC-KRAB-dCas9

    Plasmid
    #120355
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120355-plasmid-map-sequence-id-237262

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  35. pSP569

    Plasmid
    #139499
    Purpose
    Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pSTL1 24xPP7sl STL1 100 - 600
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pSTL1
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139499-plasmid-map-sequence-id-292017

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Showing: 10401 - 10450 of 32228 results