We narrowed to 7,520 results for: Ski;

1. pRS413-TEF1pr-SEC7-FAPoptim

   Plasmid

   #221124

   Purpose

   FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   SEC7-FAPoptim
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS413-TEF1pr-VPH1-FAPoptim

   Plasmid

   #221125

   Purpose

   FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   VPH1-FAPoptim
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS415-TEF1pr-MFA-NFAPoptim

   Plasmid

   #221090

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJET1.2-OsMac3

   Plasmid

   #218542

   Purpose

   Vector pJET1.2 contains the 158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA.

   Depositor

   Kirill Demchenko

   Article

   Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do

   Insert

   158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA.
   

   Use

   Synthetic Biology

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRAR101

   Plasmid

   #185046

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Expression

   Bacterial

   Mutation

   Amino acids 2-8 deleted of L31p coding region

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRAR111

   Plasmid

   #185047

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Expression

   Bacterial

   Mutation

   Last 8 amino acids deleted of L31p coding region

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRAR051

   Plasmid

   #185029

   Purpose

   400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Expression

   Bacterial

   Mutation

   Nucleotides 47-51 & 80-84 mutated rpmE 5'…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRAR052

   Plasmid

   #185030

   Purpose

   400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Expression

   Bacterial

   Mutation

   Nucleotides 56-61 and 68-73 mutated in rpmE 5…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRAR064

   Plasmid

   #185031

   Purpose

   400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Expression

   Bacterial

   Mutation

   Nucleotides of rpmE 5'UTR scrambled, Only fi…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRAR102

    Plasmid

    #185033

    Purpose

    400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    U54C mutation of rpmE 5'UTR, Only first 90 n…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRAR106

    Plasmid

    #185036

    Purpose

    400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    G79U mutation of rpmE 5'UTR, Only first 90 n…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRAR116

    Plasmid

    #185040

    Purpose

    400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    U58C and U70C of rpmE of 5'UTR, Only first 9…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRAR017-L31-sfGFP

    Plasmid

    #185024

    Purpose

    400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    Only first 90 nt of rpmE coding present

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRAR039

    Plasmid

    #185025

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    Nucleotides 1-31 deleted from rpmE 5'UTR, On…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRAR040

    Plasmid

    #185026

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    Nucleotides 35-46 deleted from rpmE 5'UTR, O…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAR041

    Plasmid

    #185027

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Expression

    Bacterial

    Mutation

    Nucleotides 47-54 & 76-86 deleted from rpmE …

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET151-GyrA_F164R238

    Plasmid

    #216230

    Purpose

    S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed arginine 238 to serine, phenylalanine 168…

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET151-GyrB_A467

    Plasmid

    #216234

    Purpose

    S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed alanine 467 to valine

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET151-GyrB_T522

    Plasmid

    #216235

    Purpose

    S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed threonine 522 to isoleucine

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRG4 CTF-FLAG

    Plasmid

    #217702

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG4 (ADGRG4 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2721

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge