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Showing: 801 - 840 of 2761 results

  1. pSTAP-seq_human-4xUAS

    Plasmid
    #125150
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    125150-plasmid-map-sequence-id-254778

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  3. pJL1-TTlight-4xDQNAT

    Plasmid
    #128394
    Purpose
    In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    T7
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128394-plasmid-map-sequence-id-260189

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  7. pAGW-dpse-GAL4-DBD

    Plasmid
    #125153
    Purpose
    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    Use
    Gateway fly expression vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125153-plasmid-map-sequence-id-248016

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  8. pSTAP-seq_fly-zfh1

    Plasmid
    #86381
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86381-plasmid-map-sequence-id-173935

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  11. pSTAP-seq_fly-tj

    Plasmid
    #86386
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster tj enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86386-plasmid-map-sequence-id-173933

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  14. pSTAP-seq_fly-4xUAS

    Plasmid
    #125149
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125149-plasmid-map-sequence-id-247994

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  17. pSTAP-seq_fly-ssp3

    Plasmid
    #86382
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ssp3 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86382-plasmid-map-sequence-id-173943

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  18. pSTAP-seq_fly-ham

    Plasmid
    #86383
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ham enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86383-plasmid-map-sequence-id-173939

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  19. pSTAP-seq_fly-sgl

    Plasmid
    #86384
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster sgl enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86384-plasmid-map-sequence-id-173940

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  20. pSTAP-seq_fly-ncm

    Plasmid
    #86385
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ncm enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86385-plasmid-map-sequence-id-173945

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  21. pJK193

    Plasmid
    #71834
    Purpose
    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71834-plasmid-map-sequence-id-131053

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  33. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Use
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  35. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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  40. pSTAP-seq_fly-zfh1_spikeIn-1

    Plasmid
    #86387
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
    Use
    Stap-seq spike in vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86387-plasmid-map-sequence-id-193668

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Showing: 801 - 840 of 2761 results