Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 1,640 results for: plasmid for e coli

Showing: 1001 - 1050 of 1640 results

  1. pTS2355_Tier3(PB)-iRFP_PuroR

    Plasmid
    #169654
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169654-plasmid-map-sequence-id-365633

    Enlarge

  13. pGEX6P1 pS43 METTL3

    Plasmid
    #200732
    Purpose
    Bacterial expression of GST-Tagged codon optimized human pS43 METTL3
    Depositor
    Claudio Alarcón
    Insert
    pS43 METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli, UAG co…
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200732-plasmid-map-sequence-id-401809

    Enlarge

  14. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

    Enlarge

  15. pUCX

    Plasmid
    #60681
    Purpose
    E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression
    Depositor
    David Ackerley
    Article
    Prosser et al Biotechnol Lett. 2014 Sep 26.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60681-plasmid-map-sequence-id-108962

    Enlarge

  16. pVS2

    Plasmid
    #33144
    Depositor
    Michael Rosbash
    Article
    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.
    Inserts
    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    Use
    Tags
    Expression
    Yeast
    Mutation
    GFP is mutated such that amino acids 64-73 (FSYGV…
    Promoter
    bidirectional GAL1-GAL10 promoter
    Available since
    Dec. 20, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. pTH847-gst-optsc

    Plasmid
    #96908
    Purpose
    Plasmid for yeast expression of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96908-plasmid-map-sequence-id-206073

    Enlarge

  20. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

    Enlarge

  31. pTH846-gst-dissc

    Plasmid
    #96907
    Purpose
    Plasmid for yeast expression of GST encoded by non-preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon dis-optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96907-plasmid-map-sequence-id-206054

    Enlarge

  33. pRMC2

    Plasmid
    #68940
    Purpose
    tetracycline-inducible expression vector for Staphylococcus aureus
    Depositor
    Tim Foster
    Article
    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1
    Type
    Empty backbone
    Use
    Tet inducible e. coli/staphylococcus shuttle vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    68940-plasmid-map-sequence-id-171245

    Enlarge

  35. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

    Enlarge

  46. Sc RFC[hk-1s + 5]-pLANT/RIL

    Plasmid
    #239200
    Purpose
    Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC1 (RFC1 Budding Yeast)
    RFC5 (RFC5 Budding Yeast)
    Use
    Tags
    His-10 + PKA kinase motif on truncated Sc RFC1
    Expression
    Bacterial
    Mutation
    N-terminal truncation
    Promoter
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239200-plasmid-map-sequence-id-476583

    Enlarge

  47. pGEX-4T_CIP2A_1-560_V5His

    Plasmid
    #217922
    Purpose
    E. coli expression of GST-tagged CIP2A_1-560 protein, with C-terminal V5 His tag
    Depositor
    Jukka Westermarck
    Insert
    CIP2A (CIP2A Human)
    Use
    Tags
    GST and V5-His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217922-plasmid-map-sequence-id-433893

    Enlarge

  48. His 3C MKK3 42-347

    Plasmid
    #87879
    Purpose
    E. coli expression of MKK3 aa 42-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 42-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87879-plasmid-map-sequence-id-174922

    Enlarge

  49. His 3C MKK6 1-334

    Plasmid
    #87881
    Purpose
    E. coli expression of MKK6 aa 1-334
    Depositor
    Peter Mace
    Insert
    MKK6 (MAP2K6 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-334
    Promoter
    T7
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87881-plasmid-map-sequence-id-174845

    Enlarge

Showing: 1001 - 1050 of 1640 results