We narrowed to 443 results for: ADK

1. pJS-BCD-Strep-PC-σ

   Plasmid

   #214037

   Purpose

   Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PC-σ
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

   Plasmid

   #166814

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

   Plasmid

   #166811

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   LL1193EE, P1214R, K1277E

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

   Plasmid

   #166808

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K1277E

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

   Plasmid

   #166807

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H1276G

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

   Plasmid

   #166812

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H1276G

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

   Plasmid

   #166813

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K1277E

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

   Plasmid

   #164002

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1461R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A

   Plasmid

   #164001

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W1460A

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A

    Plasmid

    #164000

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    M1457A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A

    Plasmid

    #163999

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Y1445A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E

    Plasmid

    #163998

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R1427E

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R

    Plasmid

    #163997

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1414R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R

    Plasmid

    #163996

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1411R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R

    Plasmid

    #163994

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1397R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E

    Plasmid

    #163993

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R1392E

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E

    Plasmid

    #163992

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R1386E

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNon-target

    Plasmid

    #214053

    Purpose

    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Non-target sequence
    

    Use

    Non-target rna plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid

    #216156

    Purpose

    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSAVED-CHAT-cbm_PCaspase

    Plasmid

    #214047

    Purpose

    Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    lacUV5 promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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