We narrowed to 2,540 results for: EXO

1. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-lambda red operon

   Plasmid

   #165521

   Purpose

   Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   lambda red operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

   Plasmid

   #208942

   Purpose

   The CE1 construct, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

   Plasmid

   #217798

   Purpose

   Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR and Lentiviral

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   EF1a

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

   Plasmid

   #208943

   Purpose

   A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

   Plasmid

   #208944

   Purpose

   A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

   Plasmid

   #208969

   Purpose

   Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid

    #217801

    Purpose

    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid

    #208956

    Purpose

    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid

    #217804

    Purpose

    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid

    #208959

    Purpose

    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid

    #208947

    Purpose

    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

    Plasmid

    #208949

    Purpose

    A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

    Plasmid

    #208948

    Purpose

    A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge