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Showing: 401 - 420 of 550 results
  1. pCW-eGFP-FHA-SHLD2-m1

    Plasmid
    #114122
    Purpose
    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Insert
    SHLD2 (m1 mutant) (SHLD2 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    RNF8 FHA domain (aa 2-160) and eGFP
    Expression
    Mammalian
    Mutation
    W489A, F494A, W495A mutations introduced
    Promoter
    TRE promoter, Tet ON
    Available since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Insert
    PP7 coat protein optimized
    Use
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Promoter
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
  3. P22_wtCP_SP141his

    Plasmid
    #122651
    Purpose
    Expression of P22 virus like particle in bacteria
    Depositor
    Insert
    P22 Coat: WT / SP: 6xHis-SP141
    Use
    Tags
    Expression
    Bacterial
    Mutation
    truncated SP, P120H in the capsid scaffold (see …
    Promoter
    Available since
    Aug. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid
    #192714
    Purpose
    Mammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag
    Depositor
    Insert
    MCP-ADAR2-DD(E488Q/T490A)-V5
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    E488Q/T490A
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid
    #192713
    Purpose
    Gateway entry vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag
    Depositor
    Insert
    MCP-ADAR2-DD(E488Q/T490A)-V5
    Use
    Gateway entry vector
    Tags
    V5
    Expression
    Mutation
    E488Q/T490A
    Promoter
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pTU1-D-spsCD

    Plasmid
    #112788
    Purpose
    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Insert
    spsCD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pTU1-A-spsCU

    Plasmid
    #112787
    Purpose
    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Insert
    spsCU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid
    #131134
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Insert
    NLS-HA-MCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pSX2719

    Plasmid
    #200763
    Purpose
    Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.
    Depositor
    Insert
    MCP-24xGCN4
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    semo-1
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 401 - 420 of 550 results