We narrowed to 1,268 results for: abo.1

1. pET-His6-en15dFnCas9GFP

   Plasmid

   #201951

   Purpose

   Expression of en15dFnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Expression of en15dFnCas9-GFP in bacterial cells
   

   Use

   CRISPR

   Tags

   6x His, HA, NLS, and mEGFP

   Expression

   Bacterial

   Mutation

   E1603H on FnCas9

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSirt3-RFP

   Plasmid

   #216806

   Purpose

   Human Sirt3-RFP driven by the CaMKII promoter

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

   Insert

   Sirt3 (SIRT3 Human)
   

   Use

   Lentiviral

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEAST-J23101-sfGFP

   Plasmid

   #116917

   Purpose

   Strong constitutive expression of super-folder GFP for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   J23101

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEAST-pBen-Luc

   Plasmid

   #122694

   Purpose

   Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   Firefly luciferase
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEAST_J23101-bphC

   Plasmid

   #128137

   Purpose

   The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   bphC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEAST-pBen-sfGFP

   Plasmid

   #114598

   Purpose

   "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   PBen

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEAST-BenR

   Plasmid

   #114597

   Purpose

   "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   BenR
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRTetR-GAL80

   Plasmid

   #185853

   Purpose

   Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR sgSLC19A1

    Plasmid

    #102314

    Purpose

    genetic depletion of SLC19A1

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgSLC19A1 (SLC19A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP4D41

    Plasmid

    #185841

    Purpose

    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pILGFP10CF72 (B11)

    Plasmid

    #185859

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP8EFA

    Plasmid

    #185854

    Purpose

    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    DHFR P66L

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEAST_J23101-bphD

    Plasmid

    #128138

    Purpose

    The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    bphD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. TetO-FUW-PBRM1-pgk-puro

    Plasmid

    #85746

    Purpose

    tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

    Depositor

    Emily Dykhuizen

    Article

    Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.23-Fosprom

    Plasmid

    #188113

    Purpose

    Expresses luciferase under control of Fos promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

    Insert

    Fos promoter (Fos Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fos endogenous promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pESC-URA-hPOLISc

    Plasmid

    #60819

    Purpose

    to express human Pol iota recoded to yeast codons in yeast

    Depositor

    Youri Pavlov

    Article

    Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

    Insert

    POLI (POLI Human)
    

    Use

    Tags

    c-myc

    Expression

    Yeast

    Mutation

    optimized to yeast codon usage

    Promoter

    GAL1

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CFP(159-238) in pcDNAI/Amp

    Plasmid

    #60896

    Purpose

    This vector attaches CFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    CFP(159-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn-164 to His

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPomyces-2BD

    Plasmid

    #234428

    Purpose

    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified Cas9 with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEUK037

    Plasmid

    #180829

    Purpose

    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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