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pIG-832_NY-ESO-1_TCR_FAS/4-1BB

Plasmid

#207495

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

FAS/4-1BB, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-834_NY-ESO-1_TCR_FAS/IL4R

Plasmid

#207497

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

FAS/IL4R, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-preSci site-CD45)

Plasmid

#177889

Purpose

Baculo expression of GST tag fused to human CD45 ICD

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

CD45 (aa 598-1304 only) (PTPRC Human)

Use

Tags

TEV-GST-PreScission cut site

Expression

Insect

Mutation

L652P

Promoter

Polyhedrin

Available since

Aug. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-GFP-dynein-2(D1091–Q4307)

Plasmid

#64064

Purpose

Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

Depositor

Andrew Carter

Article

Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

Insert

Cytoplasmic dynein-2 (DYNC2H1 Human)

Use

Tags

GFP, His tag, and ZZ tag

Expression

Insect

Mutation

Deleted amino acids 1-1090, extra C-terminal vali…

Promoter

Polyhedrin

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pIG-836_NY-ESO-1_TCR_FAS/OX40

Plasmid

#207499

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

FAS/OX40, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-833_NY-ESO-1_TCR_FAS/CD28

Plasmid

#207496

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

FAS/CD28, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag FASTK

Plasmid

#20485

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

FASTK (FASTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Flag-Tipin-L195A

Plasmid

#23120

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag- and His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
ER-mit RspA-splitFAST (lentiviral plasmid)

Plasmid

#233604

Purpose

Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-splitFAST

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV/SV40

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

Plasmid

#223764

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 soluble part (BNIP3 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-rDPP9

Plasmid

#217326

Purpose

Purification of rat DPP9 from insect cells

Depositor

Yifan Wang

Insert

DPP9

Use

Tags

His-TEV

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-GCaMP6f-WPRE

Plasmid

#141237

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Expression

Mutation

Promoter

Human elongation factor-1 alpha (EF-1 alpha)

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-TEV-mCherry-TBK1

Plasmid

#198033

Purpose

Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV and mCherry

Expression

Insect

Mutation

E696K

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB MBP-SARM1 A600G

Plasmid

#195764

Purpose

Purification of SARM1 from insect cells

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Use

Tags

HIS-MBP-TEV

Expression

Insect

Mutation

A600G

Promoter

polyhedrin

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-6His-KIF1C_R169W-GFP

Plasmid

#185979

Purpose

Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Use

Tags

6xHis and eGFP

Expression

Insect

Mutation

R169W - mutation causing HSP (SPG58)

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-6His-KIF1C_P176L-GFP

Plasmid

#185978

Purpose

Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Use

Tags

6xHis and eGFP

Expression

Insect

Mutation

P176L - mutation causing HSP (SPG58)

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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