Addgene

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mTurquoise2-3xrGBD

Plasmid

#176100

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-2xrGBD

Plasmid

#176109

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-wGBD-mTurquoise2-wGBD

Plasmid

#176136

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb Wnt3 promoter-EGFP

Plasmid

#203792

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

EGFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

4kb Wnt3 promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RHOG-QL-deltaCAAX

Plasmid

#176096

Purpose

Constitutively active, nuclear localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Use

Tags

Expression

Mammalian

Mutation

QL

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEntra1a-mTurquoise2-3xrGBD

Plasmid

#176137

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_4_022_aac6-aph2-marker

Plasmid

#235898

Purpose

aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Insert

aac6-aph2-marker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-RhoG-QL-deltaCaaX

Plasmid

#176122

Purpose

Constitutively active, mitochondiral localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Use

Tags

Expression

Mammalian

Mutation

QL

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-HISX6-TEV-RSP4

Plasmid

#176693

Purpose

Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP4

Use

Tags

HISX6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP5

Plasmid

#176694

Purpose

Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP5 (RSP5 Chlamydomonas reinhardtii)

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pBR9-genomic_RSP6-HAX3

Plasmid

#176703

Purpose

Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Use

Tags

HAX3

Expression

Plant

Mutation

None

Promoter

rbcs2

Available since

Availability

Academic Institutions and Nonprofits only

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EGFR-mApple

Plasmid

#203773

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

EGFR (EGFR Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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EGFR-mEGFP

Plasmid

#203774

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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mNeongreen-aGBD(anillin)

Plasmid

#129633

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding domain of anillin (712-786)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-3xrGBD

Plasmid

#176091

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TOMM20-mTurquoise-Rac1-G12V-deltaCaaX

Plasmid

#176124

Purpose

Constitutively active, mitochondrial localized Rac1

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RAC1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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