We narrowed to 2,712 results for: nags

1. pCMVlux-cp157Venus

   Plasmid

   #197584

   Purpose

   BRET lux luciferase with cp157Venus for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   human codon-optimized Lux-cp157Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. B-gTEMP/pRSET-b

   Plasmid

   #188446

   Purpose

   B-gTEMP for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Sirius/pRSETB

   Plasmid

   #51956

   Purpose

   bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

   Insert

   Sirius
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. luxB:Venus+luxA/pRSETB Duet

   Plasmid

   #197582

   Purpose

   BRET lux luciferase with Venus for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxBVenus
   

   Use

   E.coli expression

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mSECFP/pcDNA3

   Plasmid

   #51951

   Purpose

   mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

   Insert

   mSECFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. luxA+luxB/pRSETB Duet

   Plasmid

   #197581

   Purpose

   Expressing luxA and luxB under dual T7 promoter

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxB
   

   Use

   Tags

   T7 tag and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-NCad-GA

   Plasmid

   #191666

   Purpose

   ddGFP-A-inserted mouse N-cadherin

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

   Insert

   N-cadherin
   

   Use

   Tags

   HA and ddGFP-A

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDAI-SceI

   Plasmid

   #32061

   Purpose

   Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Insert

   I-SceI nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pdhfr

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11a+CYP119

   Plasmid

   #66131

   Purpose

   Expresses the wild type of CYP119

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-NCad-B1

    Plasmid

    #191667

    Purpose

    ddFP-B1-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B1

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-NCad-V

    Plasmid

    #191665

    Purpose

    mVenus-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SCP1-CMVenh-luc

    Plasmid

    #22216

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP1 (super core promoter 1)-CMVenh luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. msEGFP-PACR_pcDNA3

    Plasmid

    #55774

    Purpose

    Genetically encoded caged Ca2+

    Depositor

    Takeharu Nagai

    Article

    Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

    Insert

    PACR
    

    Use

    Tags

    msEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-NCad-B3

    Plasmid

    #191668

    Purpose

    ddFP-B3-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B3

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. vgCam-pRSETb

    Plasmid

    #193362

    Purpose

    A FRET-based calcium probe using Sumire

    Depositor

    Takeharu Nagai

    Article

    Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

    Insert

    vgCam
    

    Use

    E.coli expression

    Tags

    His X6 tag and Strep tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBHPS-SortA

    Plasmid

    #21930

    Depositor

    Fuyuhiko Inagaki

    Article

    Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

    Insert

    sortase
    

    Use

    Tags

    GB1, HRV3C cleagage site, and His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

    Plasmid

    #18017

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V ECFP-W67G
    

    Use

    Tags

    ECFP-W67G and Venus

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional ECFP

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Kohinoor/pRSETb

    Plasmid

    #67770

    Purpose

    Kohinoor for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

    Plasmid

    #18016

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V Venus-Y67G
    

    Use

    Tags

    ECFP and Venus-Y67G

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional Venus

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. msEGFP-PACR_pRSETB

    Plasmid

    #55775

    Purpose

    Genetically encoded caged Ca2+

    Depositor

    Takeharu Nagai

    Article

    Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

    Insert

    PACR
    

    Use

    Tags

    6xHis and msEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 3, 2014

    Availability

    Academic Institutions and Nonprofits only