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mc2 hygro EF1a

Plasmid

#206222

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro

Plasmid

#206216

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

Plasmid

#169588

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH255-Tier1-OTetO7-PCMVmin1-SEAP

Plasmid

#169587

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-1

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH72-Tier1-PhCMV-SEAP

Plasmid

#169560

Purpose

Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven human secreted placental alkaline phosphatase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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LUC56 (Luc/UGA/SP)

Plasmid

#112147

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)

Use

Tags

Expression

Mammalian

Mutation

Cysteine 258mutated to selenocysteine (U)

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pVH228-Tier1-OVanO2-PCMVmin2-SEAP

Plasmid

#169564

Purpose

Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP production

Use

Tags

Expression

Mammalian

Mutation

Promoter

VanO2-PCMVmin

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH321-1-Tier1-PhCMV-dCas9-3xNLS

Plasmid

#169595

Purpose

Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH9-Tier1-PhCMV-3xNLS

Plasmid

#169478

Purpose

Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBBI

Plasmid

#65795

Purpose

Vector for iCre mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al Transgenic Res. 2013 Aug;22(4):873-5. do

Insert

iCre

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAB300-Tier1-PhCMV-rTetR

Plasmid

#169577

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline repressor protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2368-Tier1-PhCMV_VP64

Plasmid

#169569

Purpose

Tier-1 vector encoding PhCMV-driven VP64 transactivator domain (PhCMV-VP64-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2367-Tier1-PhCMV_VP16

Plasmid

#169568

Purpose

Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1011-Tier1-PhCMV-Nluc

Plasmid

#169545

Purpose

Tier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven NanoLuc Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2401-Tier1-PhCMV-HygroR

Plasmid

#169493

Purpose

Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven hph

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1018-Tier1-PhCMV-p2A

Plasmid

#169491

Purpose

Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven 2A sequence of porcine teschovirus-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2415-Tier1-PPGK-1xEAAAK

Plasmid

#169482

Purpose

Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven EAAAK-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2412-Tier1-PPGK-2xGS

Plasmid

#169481

Purpose

Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven GS-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH64-Tier1-PSV40-Citrine_p2a_SEAP

Plasmid

#169558

Purpose

Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PSV40-driven citrine-P2A-SEAP fusion construct

Use

Tags

Expression

Mammalian

Mutation

Promoter

PSV40

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA -CLCN3-3xFlag

Plasmid

#225506

Purpose

CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

CLCN3 (CLCN3 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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