We narrowed to 8,361 results for: sgrna

1. pSEVA26_sgRNA-A0_M13ps

   Plasmid

   #231413

   Purpose

   M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   sgRNA-A0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA19_LacI_pLac-sgRNA-1

   Plasmid

   #231408

   Purpose

   sgRNA-1 expression from an IPTG-inducible promoter.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   sgRNA-1
   LacI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA19_AraC_pBAD-sgRNA-1

   Plasmid

   #231407

   Purpose

   sgRNA-1 expression from an arabinose-inducible promoter.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   sgRNA-1
   AraC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVA26_sgRNA-A1_M13ps

   Plasmid

   #231414

   Purpose

   M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-A1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVA19_sgRNA-C0_M13ps

   Plasmid

   #231410

   Purpose

   M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEVA19_sgRNA-1_M13ps

   Plasmid

   #231409

   Purpose

   M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   sgRNA-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-sgRNA_MET_uORF1

   Plasmid

   #216537

   Purpose

   gRNA and template for MET 5'UTR uORF1 mutation

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

   Insert

   pegRNA MET 5'UTR uORF1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-sgRNA_MET_uORF2

   Plasmid

   #216538

   Purpose

   gRNA and template for MET 5'UTR uORF2 mutation

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

   Insert

   pegRNA MET 5'UTR uORF2 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p103DestTol2pA2-4xU6sgRNA

   Plasmid

   #227778

   Purpose

   Destination vector with U6 driven 4 sgRNAs

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Insert

   4xU6:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA1_Cas9-hGem_Smc4

    Plasmid

    #217667

    Purpose

    expresses Cas9-hGem and guideRNA for tagging of SMC4

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2 NT sgRNA5

    Plasmid

    #164930

    Purpose

    Expresses Non-targeting sgRNA5 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA5 (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SHLD3 KO sgRNA #1

    Plasmid

    #207105

    Purpose

    pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CGCTATCAAGATTTATACCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HaloTag KO sgRNA

    Plasmid

    #207102

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTCGATGTTGGTCCGCGCGA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SHLD3 KO sgRNA #2

    Plasmid

    #207106

    Purpose

    pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CTGAAGGAACAGACTAATTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 53BP1 KO sgRNA

    Plasmid

    #207104

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGATTCTCAGCCTGAAAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6-sgRNA-hcr3

    Plasmid

    #193660

    Purpose

    Expression of tandem pre-sgRNA array hcr3 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6-sgRNA-hcr5

    Plasmid

    #193661

    Purpose

    Expression of tandem pre-sgRNA array hcr5 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6-sgRNA-pcr4

    Plasmid

    #193662

    Purpose

    Expression of tandem pre-sgRNA array pcr4 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LENTICRISPR-LUC7L2_sgRNA1

    Plasmid

    #201602

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LENTICRISPR-PGM2_sgRNA2

    Plasmid

    #201617

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    PGM2 (PGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge