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Showing: 401 - 420 of 1395 results

  2. pMSP1E2D1

    Plasmid
    #161606
    Purpose
    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E2D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161606-plasmid-map-sequence-id-318420

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  5. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Use
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

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  6. pMSP1E1D1

    Plasmid
    #161605
    Purpose
    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E1D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161605-plasmid-map-sequence-id-317650

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  8. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Randy Poon
    Article
    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a promoter and RPBSA promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171683-plasmid-map-sequence-id-338224

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  10. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  16. pNR291

    Plasmid
    #79555
    Purpose
    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    GFPmut3b
    mTagBFP
    mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79555-plasmid-map-sequence-id-148766

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  17. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO, native, native (respectively)
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  18. pPTK002-2-pGAP

    Plasmid
    #84961
    Purpose
    Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter
    Depositor
    Volker Sieber
    Article
    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102
    Insert
    Glyceraldehyde-3-phosphate dehydrogenase promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    BsaI site removed (1805 c to g)
    Promoter
    Non
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    84961-plasmid-map-sequence-id-173359

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Showing: 401 - 420 of 1395 results