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PCDNA3-N3FLAG-MYB-(1-327)

Plasmid

#105612

Purpose

transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

N terminal fragment of MYB (mouse), amino acids 1-327 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-DBD(1-200)

Plasmid

#105610

Purpose

transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

DBD fragment of MYB (mouse), amino acids 1-200 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3HA-TAF12-HFD(50-130)

Plasmid

#105613

Purpose

transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 HFD amino acid 50-130 with HA tag (Taf12 Mouse)

Tags

3*HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TAF4(827-909)

Plasmid

#105614

Purpose

transient express TAF4 HFD in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD amino acid 827-909 (Taf4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARPT-334-406-Hygro

Plasmid

#238099

Purpose

Expression of structurally predicted WWE domain of PARPT with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARPT

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 334-406 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-ZCCHV-594-684-Hygro

Plasmid

#238102

Purpose

Expression of structurally predicted WWE domain of ZCCHV with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

ZCCHV

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 594-684 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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α-synuclein-3H

Plasmid

#205715

Purpose

Mammalian expression of alpha-synuclein-3H

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-αS-2

Plasmid

#205706

Purpose

Mammalian expression of alpha-synuclein tandem dimer

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein tandem dimer (SNCA Human)

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRSCR

Plasmid

#185345

Purpose

Expression of soluble G2BR with scrambled sequence

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

scrambled gp78 G2BR sequence

Tags

EGFP

Expression

Mammalian

Mutation

scrambled sequence

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSA

Plasmid

#195207

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

S162/339/340A

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

S162/339/340D

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1/2*

Plasmid

#195211

Purpose

Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

Dual PDZ1/2 GYGF>GYAA motif mutant

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-GgVCL/*TBM

Plasmid

#162790

Purpose

MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Mutated Gallus gallus vinculin (G454V) (VCL Chicken)

Tags

IRES-GFP

Expression

Bacterial and Mammalian

Mutation

changed Gly 454 to Val

Promoter

beta actin

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shTAF12.364

Plasmid

#105572

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MYB-TAD(251-327)

Plasmid

#105619

Purpose

bacterially express GST tagged MYB TAD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB-TAD domain (Myb Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 251-327

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-TAF12-HFD(50-130)

Plasmid

#105615

Purpose

transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12-HFD amino acid 50-130 with FLAG tag (Taf12 Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LENC-shTAF12.364

Plasmid

#105579

Purpose

retrovirally express TAF12 shRNA with Neo resistance and mCherry marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #364

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TADA1-HFD(140-230)

Plasmid

#105984

Purpose

transient express TADA1-HFD fragment in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1-HFD (140-230) (Tada1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCS-flt4um159-HA

Plasmid

#67704

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

flt4 (flt4 Zebrafish)

Tags

HA

Expression

Mammalian

Mutation

um159

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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