Addgene

We narrowed to 9,565 results for: Ada;

Showing: 4181 - 4200 of 9565 results

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pTW120

Plasmid

#115947

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR SapI/BsaI Mut

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW081

Plasmid

#115929

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/3 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW082

Plasmid

#115930

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW083

Plasmid

#115931

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/14 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Use

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 313

Plasmid

#98586

Purpose

pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 314

Plasmid

#98584

Purpose

pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Inserts

CsrA
csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-MAGE-112-429

Plasmid

#89128

Purpose

mammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)

Plasmid

#86922

Purpose

To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-157 is deleted

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TAC-WTSI

Plasmid

#80486

Purpose

piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

WTSI

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K89R

Plasmid

#78808

Purpose

To overexpress -RIPK3 K89R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K89R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K501R

Plasmid

#78810

Purpose

To overexpress RIPK3 K501R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K501R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K363R

Plasmid

#78809

Purpose

To overexpress RIPK3 K363R in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K363R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Ubl4A K48R

Plasmid

#61809

Purpose

expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K48R

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
Danio rerio cryaa V62T

Plasmid

#47630

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Changed Valine 62 to Threonine

Promoter

T7

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa T147V

Plasmid

#47628

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Changed Theronine 147 to Valine

Promoter

T7

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa C143S

Plasmid

#47619

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Cysteine 143 to Serine

Promoter

T7

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pET21-MRPL13

Plasmid

#31344

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L13 mature form (MRPL13 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Mature form after removal of the predicted mitoch…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
mycUb-20aa

Plasmid

#20255

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin-20 amino acid extension (RBX1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-281&390-428.M231R

Plasmid

#15958

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Escherichia coli surA delta PPIase domain 2

Use

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted; M231R mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only