Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,400 results for: Coli

Showing: 6301 - 6330 of 9400 results

  1. pfMDH_D230A

    Plasmid
    #67725
    Purpose
    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed aspartate 230 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pfMDH_N191A

    Plasmid
    #67724
    Purpose
    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 191 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pfMDH_K295A

    Plasmid
    #67726
    Purpose
    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 295 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pHL 1454

    Plasmid
    #53019
    Purpose
    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlac0-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid
    #82528
    Purpose
    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1-84 with mutation of RKR70-72, K75, …
    Promoter
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82528-plasmid-map-sequence-id-156824

    Enlarge

  14. pHL 1916

    Plasmid
    #53023
    Purpose
    AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    Asp terminator and RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL 1011

    Plasmid
    #52993
    Purpose
    pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    RBS (st7)
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    pLlac0-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pHL1756

    Plasmid
    #53036
    Purpose
    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind and pConNoHindM12
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. MZB139

    Plasmid
    #217838
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    SMT3 (SMT3 Budding Yeast)
    Use
    Tags
    10x His and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217838-plasmid-map-sequence-id-432993

    Enlarge

  25. MZB164

    Plasmid
    #217842
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    HaloTag
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217842-plasmid-map-sequence-id-433000

    Enlarge

  26. pOPINF HopF2

    Plasmid
    #228397
    Purpose
    Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli
    Depositor
    Lennart Wirthmueller
    Article
    Simranjit et al bioRxiv 2023.09.25.558804
    Insert
    HopF2 (hopF2 Pseudomonas syringae pv. tomato)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    March 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228397-plasmid-map-sequence-id-465273

    Enlarge

Showing: 6301 - 6330 of 9400 results