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pLG1-puro-sgATL1-2
Plasmid#109006PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL1-sgRNA #2 (ATL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
Plasmid#101207PurposeBacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variantDepositorInsertSpCas9 variant N497A/R661A/Q695A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A and Q695APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant
Plasmid#101201PurposeBacterial expression plasmid for SpCas9, REC3 FRET variantDepositorInsertSpCas9 variant C80S/C574S/S701C/S960C
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S701C and S960CPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
Plasmid#101214PurposeBacterial expression plasmid for SpCas9 + K855A, HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/K855A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C and K855APromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant
Plasmid#101200PurposeBacterial expression plasmid for SpCas9, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C and D273CPromoterT7Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK4387 - human expression plasmid for SpCas9 Cluster 5
Plasmid#101191PurposeHuman expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster5(K918A/V922A/R925A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationK918A/V922A/R925APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3645 - human expression plasmid for SpCas9 Cluster 3
Plasmid#101187PurposeHuman expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster3(T657A/G658A/W659A/R661A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationT657A/G658A/W659A/R661APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only
