We narrowed to 88,677 results for: Mal;

1. NKP40 RFP (794)

   Plasmid

   #62006

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf32 (C17orf32 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDest40 DHFR.dn-cHSF1

   Plasmid

   #134735

   Purpose

   Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMasterK

   Plasmid

   #165081

   Purpose

   Episomal expression of reprogramming factors.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   MYC (MYC Human)
   LRH1 (NR5A2 Human)
   NANOG (NANOG Human)
   lin28 (LIN28A Human)
   EGFP
   HSV TK
   KDM4D (KDM4D Callithrix jacchus)
   GLIS1 (GLIS1 Human)
   

   Use

   Episomal

   Expression

   Mammalian

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. RN3P-KDM5B-FLAG

   Plasmid

   #86398

   Purpose

   FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

   Depositor

   Wei Xie

   Article

   Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

   Insert

   KDM5B (Kdm5b Mouse)
   

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   Lac

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-hygro-2xMyc-Rac1-P29S

   Plasmid

   #128581

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine.

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-hygro-2xMyc-Rac1-Q61L

   Plasmid

   #128582

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine.

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-hERalpha

   Plasmid

   #101141

   Purpose

   Mammalian expression of full-length human estrogen receptor-alpha

   Depositor

   Elizabeth Wilson

   Article

   Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

   Insert

   estrogen receptor-alpha (ESR1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-SpCas9 hygro

   Plasmid

   #104995

   Purpose

   This lentiviral construct delivers hSpCas9 and hygromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mAzami-Green-Galectin

    Plasmid

    #102419

    Purpose

    Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

    Depositor

    Geert van den Bogaart

    Article

    Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

    Insert

    LGALS3 (LGALS3 Human)
    

    Tags

    mAzami-green

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-SOUL-P2A-tdTomato

    Plasmid

    #153019

    Purpose

    Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    P2A-tdTomato

    Expression

    Mammalian

    Mutation

    C128S/D156A/T159C

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3. Flag-CSL WT

    Plasmid

    #167004

    Purpose

    Mammalian expression of CSL WT

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

    Plasmid

    #170226

    Purpose

    Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    IKBKB (IKBKB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    S177E, S181E

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid

    #182652

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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