We narrowed to 6,963 results for: RAP

1. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

   Plasmid

   #171812

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-gB-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

   Plasmid

   #171811

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

   Plasmid

   #171810

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

   Plasmid

   #171808

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-Ova-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-mNeon-F-gB-BlastR [M1G]

   Plasmid

   #171807

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-gB-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPaint-mNeon-F-hgp100-BlastR [M1G]

   Plasmid

   #171806

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-hgp100-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

   Plasmid

   #171804

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-Ova-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Cry2-mCherry- YAP-N-IDR

   Plasmid

   #166446

   Purpose

   Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   Cry2, mCherry, YAP-N-IDR(1-164) (YAP1, CRY2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLT3REVIR_MARK3 2573

   Plasmid

   #107233

   Purpose

   shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3 shRNA (22 nt) (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLT3REVIR_MARK3 1122

    Plasmid

    #107232

    Purpose

    shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3 shRNA (22 nt) (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

    Plasmid

    #27212

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

12. zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

    Plasmid

    #27213

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. OKSIM

    Plasmid

    #24603

    Depositor

    Jose Cibelli

    Article

    Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

    Insert

    Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Oct4-2A-KLF4-2A-Sox2-IRES-Myc

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-EF1A-MYC-IRES-lucOS

    Plasmid

    #129776

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Use

    Tags

    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid

    #128386

    Purpose

    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

    Depositor

    Craig Duvall

    Article

    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

    Inserts

    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Human, Synthetic)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    C-terminal luc2 fragment and N-terminal luc2 frag…

    Expression

    Mammalian

    Mutation

    N terminal carbohydrate recognition domain fused …

    Promoter

    EF1A and IRES

    Available since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MADR pDonor smFP-HA (dark) WPRE

    Plasmid

    #131007

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-HA WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    HA - 10 total HA epitope tages

    Expression

    Mammalian

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid

    #131006

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    V5 - 10 total of V5 epitope tag

    Expression

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid

    #130987

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-Myc WPRE
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    Myc - 10 total Myc tags

    Expression

    Mutation

    "dark" variant with GGG fluorophore ver…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid

    #217014

    Purpose

    Expression of CDK2-7 chimera in E.coli

    Depositor

    Manjeet Mukherjee

    Article

    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

    Insert

    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    

    Use

    Tags

    C3 protease cleavable GST tag

    Expression

    Bacterial

    Mutation

    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid

    #131005

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-FLAG WPRE
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    FLAG - 10 total FLAG tags

    Expression

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-EF1a-HIF2a dPA (P405A, P531A)

    Plasmid

    #232954

    Purpose

    Stably express a constitutively stable version of HIF2a (HIF2a dPA)

    Depositor

    William Kaelin

    Article

    Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

    Insert

    HIF2a (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P405A, P531A

    Promoter

    EF1a

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131462

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A K27M, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131463

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A G34R, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Nsp3 Mac1 - C2 (UCSF)

    Plasmid

    #169209

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid

    #129420

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    TagBFP2-V5 tag

    Expression

    Mutation

    G12V

    Promoter

    none (3x polyA to mitigate episomal expression)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Nsp3 Mac1 - P43 (Oxford)

    Plasmid

    #169210

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge