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ArpC1_pLib

Plasmid

#173678

Purpose

ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC1 (ARPC1B Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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496 pSG5L HA RB del651 (NAAIRS)

Plasmid

#10726

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del651 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

residues 651 to 656 were replaced by NAAIRS, a fl…

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
500 pSG5L HA RB del685 (NAAIRS)

Plasmid

#10729

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del685 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

residues 685 to 690 were replaced by NAAIRS, a fl…

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pIKAT111

Plasmid

#69996

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

C1459T (R487C)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR.6

Plasmid

#69997

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

Q172H, K262R

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRR-S1

Plasmid

#69998

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G329T (G110V)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S2

Plasmid

#69999

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

T441C (I114T)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S4

Plasmid

#70000

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G835C (A279P)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S5

Plasmid

#70001

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

T548G (V183G)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S6a

Plasmid

#70002

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

T637C (F213L)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S7

Plasmid

#70003

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G758A (R253H)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S8

Plasmid

#70004

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

C1459A (R487S)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pIKAT127

Plasmid

#70005

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G444T (E148D)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-E3HCA8

Plasmid

#103100

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBacPAK9_WT-LGP2

Plasmid

#248165

Purpose

Expresses full-length human LGP2 in sf9 insect cells

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-SV40-pA (JDW 1417)

Plasmid

#242570

Purpose

Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCherry stop SV40 pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK58K_6G7

Plasmid

#177302

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW17K_6G5

Plasmid

#177285

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222-SIN3A-3xFLAG

Plasmid

#109213

Purpose

Encodes human full-length SIN3A with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3A (SIN3A Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-HDAC1

Plasmid

#109209

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Synthetic, Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Article

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-D3-ASK1

Plasmid

#226619

Purpose

Co-expression of D3 and ASK1 complex in insect cells

Depositor

Ning Zheng

Article

Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

Inserts

D3
ASK1 (SKP1 Mustard Weed)

Tags

His tag followed with three copies of Msyb

Expression

Insect

Mutation

The flexible loop in D3 (Q478-D515) can be remove…

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mClover3-pA (JDW 1318)

Plasmid

#224530

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mClover3-pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-TTBL

Plasmid

#115233

Purpose

Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)

Expression

Mammalian

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37I-A44L

Plasmid

#162583

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

A37I and A44L

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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