We narrowed to 6,801 results for: CAR

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pMC0x_gRNA_Pos5b1_Ptet

Plasmid

#179341

Purpose

gRNA Position Vector 5b1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a2_Ptet

Plasmid

#179340

Purpose

gRNA Position Vector 5a2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAW8-aid-3HA-eDSR-Turg1:kanMX6

Plasmid

#169363

Purpose

C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Tags

3XHA

Expression

Yeast

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW8-aid-5Myc-eDSR-Turg1:kanMX6

Plasmid

#169364

Purpose

C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Tags

5XMyc

Expression

Yeast

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1927

Plasmid

#163093

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgUroD v4

Plasmid

#158036

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

UroD sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFE-vcDAB

Plasmid

#133004

Purpose

pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

vcdabB
vcdabA

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGST1-yHse1-UIM

Plasmid

#44429

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yHse1-UIM (150-190) (HSE1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
PCIN4-PURO-K8

Plasmid

#44626

Depositor

Robert Oshima

Insert

K8 (KRT8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS1529

Plasmid

#29257

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple58 (DDC Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1530

Plasmid

#29258

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple59 (DDC Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pRExT2A-CT-BmSc

Plasmid

#232208

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP Keima-OMP25

Plasmid

#224560

Purpose

expresses Keima fused to OMP25 mitochondrial localization sequence in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

Keima-OMP25 (SYNJ2BP Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-c5e-Rubicon RH

Plasmid

#216706

Purpose

Bacterial protein expression of Rubicon RH domain

Depositor

James Hurley

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

Rubicon (RUBCN Human)

Tags

MBP

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-5

Plasmid

#221479

Purpose

Gateway cloning of NKX2-5 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Gateway Cloning

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pENAAV-cTNT-actn2-BioID2-HA

Plasmid

#216831

Purpose

cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

BioID-HA (Actn2 Synthetic)

Use

AAV

Tags

HA

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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p2H12ref

Plasmid

#221188

Purpose

cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

None (same as cdGreen) and Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(HA)

Plasmid

#195022

Purpose

Gateway vector containing HA-tagged Creb5

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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