159,589 results

1. pBCCP-GFP-NLS

   Plasmid

   #173923

   Purpose

   Fluorescent labeling of the nuclear envelope

   Depositor

   Shinji Sueda

   Article

   Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

   Insert

   Biotin carboxyl carrier protein, AcGFP1
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. APOBEC3G_pLX307

   Plasmid

   #98345

   Purpose

   Lentiviral expression of APOBEC3G

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   APOBEC3G (APOBEC3G Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TORCAR(T/A)

   Plasmid

   #64928

   Purpose

   Negative-control mutant for TORCAR biosensor.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   4EBP1 domain contains T37A and T46A mutations.

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWM_12x601_25bpLinker

   Plasmid

   #157788

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_25bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. evoBE4-SpRY

   Plasmid

   #226854

   Purpose

   Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   evoBE4max-SpRY
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCS2-FlipGFP(Casp3 cleavage seq)

   Plasmid

   #124432

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq)
   

   Use

   Zebrafish (good expression)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti‐Cas9‐2A‐Blast

   Plasmid

   #73310

   Purpose

   Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG BacMam

    Plasmid

    #160451

    Purpose

    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge