We narrowed to 14,039 results for: TIM

1. pET28a(cd96)

   Plasmid

   #177901

   Purpose

   Bacterial expression of his10-tagged human cd96 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CD96 (aa 557-585 only) (CD96 Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

   Plasmid

   #115141

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Orf2mor anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO.Nfib.Hygro

   Plasmid

   #117271

   Purpose

   Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfib (Nfib Mouse)
   

   Use

   Lentiviral

   Tags

   IRES and hygromycin resistant cassette (C termina…

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. tetO.Sox9.Puro

   Plasmid

   #117269

   Purpose

   Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A and puromycin resistant cassette (C terminal …

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVT-R2Tg-WT

   Plasmid

   #238105

   Purpose

   For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Wildtype R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SspB-HaloTag-TIAM1-DHPH

   Plasmid

   #176127

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SspB-iRFP-DHPH-TIAM1

   Plasmid

   #176118

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-mCherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176138

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176135

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Entry vector

   Mutation

   See Depositor Comments Below

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEntr1a-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176134

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    U6

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p300dAIL KAT LIC 1M

    Plasmid

    #233588

    Purpose

    Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300dAIL KAT (EP300 Human)
    

    Tags

    6xHis MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1523-1554

    Promoter

    T7

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC13N-dCas9-BFP-KRAB

    Plasmid

    #127968

    Purpose

    constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-dCas9-NLS-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-SauriABE8e

    Plasmid

    #189927

    Purpose

    Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-SRRM2-mCherry

    Plasmid

    #174089

    Purpose

    Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

    Depositor

    Wesley Sundquist, Katie Ullman

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    SRRM2 (SRRM2 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Sept. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. INCTbiosyn-pUB-HspINTphiC31

    Plasmid

    #127518

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase phiC31.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TO-oNGN2

    Plasmid

    #198397

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

    Promoter

    CAG, EF1a, and TRE3G

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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