We narrowed to 13,804 results for: TIM

1. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2CG2-hsp70I:QFGal4-SV40pA

   Plasmid

   #155120

   Purpose

   Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   hsp70l:QFGal4 (hsp70l Neurospora crassa, Budding Yeast, Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   hsp70l

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2CG2-acta1:QFGal4-SV40pA

   Plasmid

   #155121

   Purpose

   Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   acta1:QFGal4
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   acta1

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

   Plasmid

   #62460

   Purpose

   expresses C151S and C288S double mutant human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteines 151 and 288 of Keap1 to respect…

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTU422

   Plasmid

   #178982

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Inserts

   gRAMP
   CRISPR array
   

   Use

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. non-targeting control gRNA (BRDN0001487130)

   Plasmid

   #80262

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183536

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   ChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   CaMKIIa

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MLKL gRNA (BRDN0001148608)

   Plasmid

   #77539

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MLKL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MLKL (MLKL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MLKL gRNA (BRDN0001146456)

   Plasmid

   #77540

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MLKL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MLKL (MLKL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ1955

    Plasmid

    #84830

    Purpose

    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized tdTomato
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. NEK7 gRNA (BRDN0001162196)

    Plasmid

    #77740

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NEK7

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NEK7 (NEK7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-flex-Clover

    Plasmid

    #135177

    Purpose

    AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. non-targeting control gRNA (BRDN0001145598)

    Plasmid

    #80173

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEA1

    Plasmid

    #176527

    Purpose

    Delivers all prime editing nickase components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174643

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
    

    Use

    Tags

    SSPB(nano)-GFP

    Expression

    Mammalian

    Mutation

    EGFP: Met1Del; ppKin14VIb(aa861-1321)

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW003-PpsbA-mNeonGreen

    Plasmid

    #205019

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #68719

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MLKL gRNA (BRDN0001149268)

    Plasmid

    #77541

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. SspB-HaloTag-DHPH-TIAM1

    Plasmid

    #176114

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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