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pBABE-zeo largeTcDNA

Plasmid

#1779

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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LgBiT-dSaCas9

Plasmid

#236758

Purpose

Split NanoLuc luciferase biosensor fused to dSaCas9

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc LgBit

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSpCas9-SmBiT

Plasmid

#236759

Purpose

Split NanoLuc luciferase biosensor fused to dSpCas9

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc SmBit

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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HaloTag-dSpCas9

Plasmid

#238946

Purpose

N-terminal fusion of HaloTag to dSpCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

HaloTag

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSaCas9-NLuc

Plasmid

#238947

Purpose

C-terminal fusion of NanoLuc luciferase to dSaCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(sfGFP)

Plasmid

#126975

Purpose

Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSGAb-km

Plasmid

#121999

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Full length.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-3xFLAG-gateway-EGFP

Plasmid

#122845

Purpose

N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_CKAR3

Plasmid

#238413

Purpose

AAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Use

AAV

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP

Plasmid

#67743

Purpose

Pr promoter expressing deGFP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_minCMV

Plasmid

#62418

Purpose

MXS_chaining vector with minCMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCHA-VRC01-scFv

Plasmid

#44874

Depositor

Dane Wittrup

Article

Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

Insert

VRC01 scFv

Tags

HA and Myc

Expression

Yeast

Promoter

GAL1

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCBL101.1

Plasmid

#199724

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

Multiple Cloning Site, NPTII

Expression

Plant

Mutation

"GAGACC" to "GATACC"

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_mutCKAR3

Plasmid

#238414

Purpose

AAV construct with CKAR3 mutant expression under a human synapsin promotor and WPRE3 translation enhancer.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Use

AAV

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTgenomic

Plasmid

#1778

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mGreenLantern

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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