We narrowed to 6,627 results for: hwa

1. pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

   Plasmid

   #224387

   Purpose

   Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   WD 6 domain deleted (aa 242-282)

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

   Plasmid

   #224385

   Purpose

   Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   WD 4 domain deleted (aa 153-195)

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

   Plasmid

   #198570

   Purpose

   SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed arginine R76 to alanine (R76A)

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IRES-blast GFP-SPNS1(E164K)

   Plasmid

   #198572

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNK845

   Plasmid

   #219746

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal luciferase
   

   Use

   Luciferase and Synthetic Biology

   Mutation

   I3S, N4T, F11L, I63T, T99P, T192S, A199P

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GID4-Nluc

   Plasmid

   #200879

   Purpose

   Mammalian expression of human GID4 with C-terminal NanoLuciferase fusion

   Depositor

   Stefan Knapp

   Article

   Owens et al bioRxiv 2023.01.17.524225

   Insert

   GID4-NanoLuciferase (C17orf39 Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBoBi-hPEN2 D90A

   Plasmid

   #183641

   Purpose

   Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   residues 90 of PEN2 mutated to alanine

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBoBi-hPEN2 2A

   Plasmid

   #183640

   Purpose

   Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   residues 35 and 40 of PEN2 mutated to alanine

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-RSLID1(WT)

   Plasmid

   #122296

   Purpose

   expresses RSL1D1 protein in mammalian cells (puro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   vector's LTR

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

    Plasmid

    #108374

    Purpose

    Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

    Plasmid

    #108372

    Purpose

    Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107578

    Purpose

    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fbp promoter Bacillus megaterium DSM319

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEAHISSSO2462

    Plasmid

    #97064

    Purpose

    Expressing Sulfolobus solfataricus orf SSO2462 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus orf SSO2462
    

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Mutation

    none

    Promoter

    T7

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEAHISSSO2452

    Plasmid

    #97059

    Purpose

    Expressing Sulfolobus solfataricus orf SSO2452 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus orf SSO2452
    

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Mutation

    none

    Promoter

    T7

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEAHISPSVORF126

    Plasmid

    #97021

    Purpose

    Expressing Pyrobaculum spherical virus orf126 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    PSV orf 126
    

    Tags

    N-ter TEV protease cleavable 6HIs

    Mutation

    none

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEAHISPA5006

    Plasmid

    #97016

    Purpose

    Expressing Pseudomonas aeruginosa orf PA5006 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    P aeruginosa orf PA5006
    

    Tags

    N-ter TEV protease cleavable 6HIs

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEHISSIRV119

    Plasmid

    #97054

    Purpose

    Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    SIRV orf 119
    

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEHISSIRV55

    Plasmid

    #97055

    Purpose

    Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    SIRV orf 55
    

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEAHISPA0856

    Plasmid

    #97014

    Purpose

    Expressing Pseudomonas aeruginosa orf PA0856 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    P aeruginosa orf PA0856
    

    Tags

    N-ter TEV cleavable 6HIs

    Mutation

    N-terminal deletion (AA 1-34)

    Promoter

    T7

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEAHISPA4631

    Plasmid

    #97017

    Purpose

    Expressing Pseudomonas aeruginosa orf PA4631 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Nucleoside-diphosphate-sugar epimerase
    

    Tags

    N-ter TEV protease cleavable 6HIs

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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