We narrowed to 5,331 results for: pcDNA3.1

1. pCDNA3-SREBP-2-GFP

   Plasmid

   #204202

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Tags

   T7x3 and eGFP

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP-GFP-CD274-NU

   Plasmid

   #121139

   Purpose

   Mammalian expression of GFP tagged CD274

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Inserts

   CD274 (CD274 Human)
   CD274 (CD274 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HiBiT-FZD8

   Plasmid

   #195846

   Purpose

   Expresses human FZD8 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD8 (FZD8 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1(-) mouse Egr2

   Plasmid

   #107997

   Purpose

   Expresses mouse Egr2 in mammalian cells

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr2 (Egr2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoV2-N-P199L

   Plasmid

   #177949

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P199L) (N )
   

   Expression

   Mammalian

   Mutation

   P199L

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rlucrarr3-Flash6

   Plasmid

   #74134

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

7. hGATA-1 WT

   Plasmid

   #118352

   Purpose

   This plasmid expresses human GATA-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rlucrarr3-Flash1

   Plasmid

   #74129

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Rlucrarr3-Flash4

    Plasmid

    #74132

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Rlucrarr3-Flash3

    Plasmid

    #74131

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Rlucrarr3-Flash2

    Plasmid

    #74130

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

13. beta Synuclein-VC

    Plasmid

    #89484

    Purpose

    expresses human beta Synuclein fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 proHB-EGF NC

    Plasmid

    #11605

    Depositor

    Rosalyn Adam

    Article

    Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

    Insert

    HB-EGF (HBEGF Human)
    

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

16. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. VN-beta Synuclein

    Plasmid

    #89483

    Purpose

    expresses human beta Synuclein fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. KRas FAK biosensor

    Plasmid

    #78301

    Purpose

    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    KRas FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3-EMTB-TurboID-V5

    Plasmid

    #190736

    Purpose

    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    ensconsin (MAP7 Human)
    

    Tags

    Myc, TurboID, and V5

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PME-1 pcDNA-StrepIII

    Plasmid

    #119292

    Purpose

    Mammalian overexpression of StrepII-tagged full length PPME1

    Depositor

    Jukka Westermarck

    Article

    Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

    Insert

    PPME1 (PPME1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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