We narrowed to 27,350 results for: sta

1. pJS-BCD-Strep-SAVED-CHAT

   Plasmid

   #214030

   Purpose

   Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS-BCD-Strep-dSAVED-CHAT

   Plasmid

   #214031

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H375A, C422A

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS-BCD-Strep-SAVED-CHAT-sm

   Plasmid

   #214033

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS-BCD-Strep-PCaspase

   Plasmid

   #214034

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUN1213 - pL0_p16OMT (pro + 5U)

   Plasmid

   #203890

   Purpose

   16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   16OMT promoter and 5'UTR
   

   Expression

   Plant

   Mutation

   Mutation of BpiI and/or BsaI cut sites for MoClo …

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

   Plasmid

   #211821

   Purpose

   PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
   

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter, U6 promoter and hPGK pr…

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1-Lamp1 FLAG

   Plasmid

   #199750

   Purpose

   Lentiviral construct to stably express Lamp1

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Lamp1 (Lamp1 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Tau Authentic Alu 10-12K317M

   Plasmid

   #194168

   Purpose

   Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 K317M
   

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET303C-hSOD1 A4V/C6S

   Plasmid

   #139667

   Purpose

   Expression plasmids containing human A4V/C6S SOD1

   Depositor

   Svetlana Antonyuk

   Article

   Chantadul et al Commun Biol. 2020 Mar 5;3(1):97. doi: 10

   Insert

   Superoxide dismutase-1 (SOD1 Human)
   

   Expression

   Bacterial

   Mutation

   change alanine 4 to valine, change cysteine 6 to …

   Promoter

   T7

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Flag-PolB(T304I)-Puro

    Plasmid

    #177143

    Purpose

    Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. wtVP1 + VP2C-GFPdeg

    Plasmid

    #166678

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. deltaVP1 + VP2C-GFPdeg

    Plasmid

    #166677

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_hE2F7mg_6ntmut

    Plasmid

    #73075

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1_hE2F7mg_10ntmut

    Plasmid

    #73076

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmirGlo-CALB2-3'UTR delta ARE&mir30

    Plasmid

    #74430

    Purpose

    Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. -161+80pCalb2/mutE2F(-69)-like

    Plasmid

    #66743

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    GCG (-69,-68,-67) to ATT

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid

    #79823

    Purpose

    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Promoter

    TetO

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Tags

    strep tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1‐hsRNASEH2BCA

    Plasmid

    #108692

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon a…

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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