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Plasmid#62061PurposeExpression of Mouse p53 (residues 1-87) in e. coliDepositorInsertp53 (Trp53 Mouse)
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IS200
Plasmid#12604DepositorInsertIS200 transposase (SSO1474 Sulfolobus solfataricus)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a BCP1 NTase
Plasmid#195245PurposeExpresses phage BCP1 NTaseDepositorInsertBCP1 phage NTase
UseTagsHis6 and SBPExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Av05 NTase
Plasmid#195243PurposeExpresses phage Av05 NTaseDepositorInsertphage Av05 NTase
UseTagsHis6 and SBPExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Pep-H
Plasmid#185782PurposeEncodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Pep-H
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialMutationPromoterpJ21306Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A774L
Plasmid#174796PurposeBacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A774L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA774L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only