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pCYP52

Plasmid

#212885

Purpose

Expresses the Herbihabitans rhizosphaerae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP39

Plasmid

#212872

Purpose

Expresses the Saccharothrix texasensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP25

Plasmid

#212858

Purpose

Expresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP04

Plasmid

#212837

Purpose

Expresses the Rhizobium grahamii LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP02

Plasmid

#212835

Purpose

Expresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP06

Plasmid

#212839

Purpose

Expresses the Rhizobium acidisoli LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

Plasmid

#118156

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMEV4

Plasmid

#110539

Purpose

The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

Depositor

William B. Whitman

Article

Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCYP01

Plasmid

#212834

Purpose

Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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sZF2

Plasmid

#43833

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF2

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
sZF1

Plasmid

#43832

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF1

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pRSETA_crGE

Plasmid

#172927

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-H-benM

Plasmid

#73925

Purpose

Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

Depositor

George Church

Article

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate biosensing apparatus

Available Since

Jan. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHDR_DDX4-T2A-tdTomato-puroTK

Plasmid

#222519

Purpose

Homology directed repair template for knocking in tdTomato reporter to DDX4.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

tdTomato

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_SYCP3_nterm

Plasmid

#222524

Purpose

Cas9/sgRNA plasmid for targeting SYCP3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, SYCP3 sgRNA (SYCP3 Human, Synthetic)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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