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CDS12_mVenus-linker

Plasmid

#136092

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mVenus(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_TP-MpSIG2

Plasmid

#136093

Purpose

Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

TP-MpSIG2, Mapoly0214s0004

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsA_spacer

Plasmid

#136075

Purpose

Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PB-HD252

Plasmid

#172656

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD252A/pegRNA-HD252B

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCk4

Plasmid

#136698

Purpose

Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly_spike-in

Plasmid

#125151

Purpose

Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

D. pseudoobscura peak #47 enhancer

Use

Stap-seq screening vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRAF-MutL(R95F)

Plasmid

#86608

Purpose

E. coli Mutl(R95F) expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL(R95F)

Use

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

R95F

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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mCm7RNA6A

Plasmid

#84367

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2 m7-gRNA6

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly-x16

Plasmid

#71505

Purpose

flySTARR-seq screening vector with x16 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-RpS12-Gateway

Plasmid

#71508

Purpose

flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Mutation

Promoter

Available since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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