We narrowed to 2,485 results for: organization Addgene

1. pCMV superYFP-AURKA K162M-mTurq2

   Plasmid

   #157773

   Purpose

   Expression of AuroraA kinase-dead biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and superYFP

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

   Plasmid

   #222288

   Purpose

   Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   6xHis, GFP, and GST

   Expression

   Bacterial

   Mutation

   stalk region only amino acids 642 to 922

   Promoter

   T7

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

   Plasmid

   #222289

   Purpose

   Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   6His and GST

   Expression

   Bacterial

   Mutation

   stalk region only amino acids 642 to 922

   Promoter

   T7

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV ShadowG-AURKA K162M-mTurq2

   Plasmid

   #157769

   Purpose

   Expression of AuroraA kinase-dead biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and shadowG

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV ShadowY-AURKA K162M-mTurq2

   Plasmid

   #157771

   Purpose

   Expression of AuroraA kinase-dead biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and shadowY

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Ad:2CMV_A2R2-N25K3

   Plasmid

   #122242

   Purpose

   Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

   Depositor

   Corey Neu

   Article

   Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

   Inserts

   C-terminal end of Nesprin-3 (Syne3 Mouse)
   Actinin alpha 2 (Actn2 Mouse)
   

   Use

   Adenoviral

   Tags

   Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB CAG SMARCA4-Venus

   Plasmid

   #153950

   Purpose

   piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge