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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4281 - 4300 of 59254 results

  6. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  8. pEN41A-T38S38

    Plasmid
    #49524
    Purpose
    Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    constitutive TetR38
    Promoter
    Ptb38
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49524-plasmid-map-sequence-id-73916

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  10. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  11. pGL2

    Plasmid
    #48742
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +386)
    Use
    Luciferase
    Mutation
    truncated promoter region (see pGL6)
    Available Since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pGL4

    Plasmid
    #48744
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1536 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +1536)
    Use
    Luciferase
    Mutation
    truncated promoter region (see pGL6)
    Available Since
    Jan. 31, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pGL1

    Plasmid
    #48741
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to -141)
    Use
    Luciferase
    Mutation
    truncated promoter region (see pGL6)
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48741-plasmid-map-sequence-id-101474

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Showing: 4281 - 4300 of 59254 results