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pTriEx-mCherry-Zdk1-RhoA Q63L

Plasmid

#81058

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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Dronpa-DEVD-TagRFPT

Plasmid

#87704

Purpose

Fluorescent biosensor for super-resolution imaging of caspase activity.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Dronpa-DEVD-TagRFPT

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-RhoA Q63L

Plasmid

#81046

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3

Plasmid

#170854

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1-Rac1 Q61L

Plasmid

#81059

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 Q61L (RHOA Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pTriEx-mVenus-Zdk1-Rac1 Q61L

Plasmid

#81137

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab4a-c-Myc

Plasmid

#79800

Purpose

Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Tags

TagRFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSCALPS-mAgo2-EGFP

Plasmid

#209935

Purpose

Lentiviral expression of mouse Argonaute-2 in mammalian cells

Depositor

Phillip Zamore

Article

Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6

Plasmid

#170857

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-CENPB-N-22

Plasmid

#55893

Purpose

Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CENPB (CENPB Human)

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mOrange2-Golgi-7

Plasmid

#57959

Purpose

Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST cyto Grx1-roGFP2

Plasmid

#64994

Purpose

for expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGPT2_5

Plasmid

#163455

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 5 targeting GPT2 (GPT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag1-3HABeta2-mEos2

Plasmid

#114187

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Tags

3HA, FLAG, and mEOS2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-Myr-PTEN

Plasmid

#206983

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

N-terminal myristoylation site

Expression

Mammalian

Promoter

tet-on promoter

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2

Plasmid

#170853

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98932

Purpose

Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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