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pJEC578

Plasmid

#174366

Purpose

dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

dCas9 fused to SYNZIP18

Expression

Bacterial

Promoter

J23150

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA HA HP1 gamma delta C

Plasmid

#24081

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

HA

Expression

Mammalian

Mutation

Missing C-terminal 25 amino acids

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01*(ΔM)

Plasmid

#80040

Purpose

Expression or G2A mutant of EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(G2A)

Tags

Myc

Expression

Mammalian

Mutation

Gly2 mutated to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K49R

Plasmid

#44750

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K49R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
Paxillin LD

Plasmid

#186150

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

D5A; D146A; D232A; D267A; D301A

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaBS-sgRNA500

Plasmid

#149615

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaBS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0026-sgRNA500

Plasmid

#149620

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAJ20

Plasmid

#14919

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

Strongyloides stercoralis Ribosomal protein small subunit

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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TOPO Venus

Plasmid

#68493

Purpose

TOPO construct expressing Venus for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

split Venus

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-Paxillin

Plasmid

#186143

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_bGHpA

Plasmid

#62425

Purpose

MXS_chaining vector with bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bovine growth hormone poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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RhoC

Plasmid

#55702

Purpose

expression of mouse RhoC

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoC (Rhoc Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
MXS_minPGK

Plasmid

#62415

Purpose

MXS_chaining vector with minPGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-3HA

Plasmid

#17373

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3HA (WNT3 Mouse, Human)

Tags

HA

Expression

Bacterial

Mutation

This gene is a fusion of the murine and human wnt…

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
MXS_CAG

Plasmid

#62420

Purpose

MXS_chaining vector with CAG

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CAG Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K19R

Plasmid

#44749

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
RhoH

Plasmid

#55697

Purpose

expression of mouse RhoH

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoH (Rhoh Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pJUMP19-J23102_P

Plasmid

#126997

Purpose

Basic Part P- Promoter (constitutive); J23102.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23102_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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