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Plasmid#30301DepositorInsertIgG Fc Designed Binder IgG1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyTagsExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
UseTagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-ZZ(1700-1751)
Plasmid#99334PurposeBacterial expression of mouse CBP ZZ domainDepositorInsertCBP ZZ domain (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-TelR15-YPet
Plasmid#49646PurposeTALEYellow-Telomere for In Vitro TranslationDepositorInsertTelR15
UseIn vitro translationTagsYPetExpressionMutationPromoterAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-HMGS
Plasmid#108945Purposeheterologous expression of HMGS of E. faecalis in E. coliDepositorInsertHMG-CoA synthase (HMGS)
UseTagsHis6-TagExpressionBacterialMutationA110GPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Cdc12-Cdc11
Plasmid#131162PurposeExpression of Septin rodsDepositorInsert6his-Cdc12 + Cdc11
UseBicistronicTagsCdc11: S-Tag and Cdc12: 6hisExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only