We narrowed to 11,687 results for: NSI

1. pYPQAT60

   Plasmid

   #223432

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT58

   Plasmid

   #223430

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQAT61

   Plasmid

   #223433

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. psiCHECK-2-Zeb2-3'UTR

   Plasmid

   #61771

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Zeb2

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Zeb2 3'UTR
   

   Use

   Luciferase

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

   Plasmid

   #230024

   Purpose

   Expresses anti-TNFalpha PAGER(FL) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-ExRai-AKAR

   Plasmid

   #118405

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-C3-beta-spectrin

   Plasmid

   #202685

   Purpose

   fluorescent protein tagged beta-spectrin

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   EGFP:SPTB(1-2364) (SPTB Human)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTlpA_mCherry_Txe/Axe

   Plasmid

   #229147

   Purpose

   Toxin-Antitoxin (Txe/Axe)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, Txe/Axe
   

   Expression

   Bacterial

   Promoter

   PtlpA (mCherry), Txe/Axe (Native)

   Available Since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT42

   Plasmid

   #223414

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRXG

    Plasmid

    #113643

    Purpose

    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUGW-VMAT2-pHluorin

    Plasmid

    #207468

    Purpose

    Lentiviral expression of VMAT2-pHluorin

    Depositor

    Robert Edwards

    Article

    Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

    Insert

    VMAT2 (Slc18a2 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTnos (GB0037)

    Plasmid

    #68188

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos terminator
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFSKm4-ATF1

    Plasmid

    #221133

    Purpose

    Formaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detection

    Depositor

    André Studart

    Article

    Dutto et al Adv Mater. 2024 Dec 10:e2412555. doi: 10

    Insert

    ATF1 (ATF1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    Pm4

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB061i

    Plasmid

    #79226

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    Ppro1; PlacIq

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. psiCHECK-2-Ywhab-3'UTR

    Plasmid

    #61782

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhab

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhab 3'UTR
    

    Use

    Luciferase

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK-2-Map3k1-3'UTR

    Plasmid

    #61776

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Map3k1

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Map3k1 3'UTR
    

    Use

    Luciferase

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. psiCHECK-2-Fhod1-3'UTR

    Plasmid

    #61773

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Fhod1

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Fhod1 3'UTR
    

    Use

    Luciferase

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. psiCHECK-2-Mapk12-3'UTR

    Plasmid

    #61783

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Mapk12

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Mapk12 3'UTR
    

    Use

    Luciferase

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

19. KillerOrange /pQE-30

    Plasmid

    #74748

    Purpose

    KillerOrange gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

    Insert

    KillerOrange
    

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    KillerRed with substitutions G3C Y66W D113S N145S…

    Promoter

    T5

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pP35S (GB0030)

    Plasmid

    #68163

    Purpose

    Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV 35S promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge