We narrowed to 14,187 results for: shi

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NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

4XFLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pUAS-MmTFP13xmyc

Plasmid

#86371

Purpose

membrane targeted Tfp1 expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

mTfp1

Use

Zebrafish

Tags

3xMyc and GAP43 membrane targeting

Promoter

UAS

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD-10xHis)

Plasmid

#176192

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

10xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcBCD)

Plasmid

#176193

Purpose

expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-PAmCherry1

Plasmid

#189718

Purpose

titratable expression of PAmCherry1 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

PAmCherry1

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Pyb1301(JBEI-11629)

Plasmid

#87959

Purpose

jStack yeast assembly into plant vectors, Level 2 pYB Vector, Hygro selection

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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CΔ1

Plasmid

#74296

Purpose

Mammalian expression of treacle (residues 1176-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1176 - 1302

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET-TALE-mCherry-15b

Plasmid

#138287

Purpose

Express tTALE-mCherry

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-mCherry

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rap1b S179AS180A

Plasmid

#118327

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b S179AS180A (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

S179AS180A

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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CΔ4

Plasmid

#74299

Purpose

Mammalian expression of treacle (residues 1271-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1271 - 1302

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-PRLH

Plasmid

#184618

Purpose

For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PRLH

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NOC

Plasmid

#184611

Purpose

For expression of a fluorescent sensor for nociceptin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NOC

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-3HA

Plasmid

#17373

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3HA (WNT3 Human, Mouse)

Tags

HA

Expression

Bacterial

Mutation

This gene is a fusion of the murine and human wnt…

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMPRMA38

Plasmid

#172937

Purpose

pQE30 encoding Wzz mutant i92

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i92 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA45

Plasmid

#172934

Purpose

pQE30 encoding Wzz mutant i32

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i32 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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