We narrowed to 12,683 results for: sequence

1. pT7-EGFP-C1-HsGIGYF1_AE

   Plasmid

   #148569

   Purpose

   Mammalian Expression of HsGIGYF1

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1 (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDual-eGFP(H66)

   Plasmid

   #63558

   Purpose

   Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(H66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. TAF15_RRM-ZNF_pGEX

   Plasmid

   #135111

   Purpose

   Recombinant expression of TAF15 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RBFOX3_RRM_pGEX

   Plasmid

   #135131

   Purpose

   Recombinant expression of RBFOX3 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBFOX3 (RBFOX3 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CyRPA-bio

   Plasmid

   #68525

   Purpose

   Expresses full-length P. vivax CyRPA ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

   Depositor

   Julian Rayner, Gavin Wright

   Article

   Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

   Insert

   CyRPA
   

   Use

   Tags

   rat CD4 d3+4, enzymatic biotinylation sequence

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP3.1-bio

   Plasmid

   #68506

   Purpose

   Expresses full-length P. vivax MSP3.1 (MSP3gamma) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

   Depositor

   Julian Rayner, Gavin Wright

   Article

   Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

   Insert

   MSP3.1
   

   Use

   Tags

   rat CD4 d3+4, enzymatic biotinylation sequence

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDN-D2irG4kwh

   Plasmid

   #44723

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   EGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. ARP-bio

   Plasmid

   #68524

   Purpose

   Expresses full-length P. vivax ARP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

   Depositor

   Julian Rayner, Gavin Wright

   Article

   Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

   Insert

   ARP
   

   Use

   Tags

   rat CD4 d3+4, enzymatic biotinylation sequence

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE60-mPU.1-ETS

    Plasmid

    #85733

    Purpose

    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

    Depositor

    Gregory Poon

    Article

    Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

    Insert

    ETS domain of transcription factor PU.1 (Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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