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hU6-sgNT1 (opti)

Plasmid

#177237

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT1

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNT2 (opti)

Plasmid

#177238

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT2

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNT3 (opti)

Plasmid

#177239

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT3

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo1 (opti)

Plasmid

#177240

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo1

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo2 (opti)

Plasmid

#177241

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo2

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo3 (opti)

Plasmid

#177242

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo3

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgLkb1_IW (opti)

Plasmid

#177243

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Lkb1 gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgLkb1_v1

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgLkb1_v2 (opti)

Plasmid

#177244

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Lkb1 gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgLkb1_v2

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpa_v1 (opti)

Plasmid

#177245

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_1st

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpa_v2 (opti)

Plasmid

#177246

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_2nd

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpb_v1 (opti)

Plasmid

#177247

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_1st

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpb_v2 (opti)

Plasmid

#177248

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_2nd

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpd_v1 (opti)

Plasmid

#177249

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_1st

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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dora[B]_rescue_construct

Plasmid

#190637

Purpose

Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Kindlin

Plasmid

#186136

Purpose

For expression of recombinant Snap-labeled kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

Plasmid

#182305

Purpose

E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH-Countdown insertion into GECO 1.2 calcium sensing domains

Tags

A modified TorA periplasmic targeting sequence pl…

Expression

Bacterial

Mutation

Spontaneous mutation of calmodulin's penulti…

Promoter

t7

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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