We narrowed to 9,775 results for: Pol

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pAAV-hSyn-ConVERGD-N2cG-WPRE

Plasmid

#218757

Purpose

Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

N2cG

Use

AAV

Mutation

See Depositor Comments

Promoter

hSyn

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-yeGFP-His6

Plasmid

#212781

Purpose

C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL

Plasmid

#218756

Purpose

Provides NOT intersectional (Cre NOT Flp) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL

Plasmid

#218755

Purpose

Provides NOT intersectional (Flp NOT Cre) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1935

Plasmid

#212315

Purpose

Express ApmFnuc protein with sumotag

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Isvmimi

Tags

6xHis-thrombin site-TwinStrepTag-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfA

Plasmid

#89616

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Electron transport flavoprotein alpha subunit

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - NFDD

Plasmid

#89624

Purpose

Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD/FAD dependent dehydrogenase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-p63RhoGEF

Plasmid

#67896

Purpose

Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-p63RhoGEF

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCA11-pMa-sgTS3-17xMS2V5-sgTS3

Plasmid

#199442

Purpose

CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

HITI donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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GFAP-Lyn-mCherry-pHluorin

Plasmid

#187980

Purpose

Membrane targeted cytoplasmic fluorescent pH sensor

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Lyn-mcherry-pHluorin

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-B/BRISBANE/60/2008

Plasmid

#187921

Purpose

Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-HA-PARP11

Plasmid

#22556

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

PARP11 (PARP11 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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BB-NIC-II-HisN-TET12

Plasmid

#45496

Depositor

Roman Jerala

Article

Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

Insert

TET12

Use

Synthetic Biology

Tags

6xHistidine tag

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pECFP-Rac1Q61L

Plasmid

#105292

Purpose

Constitutively active Rac 1

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEx-deletion-erm

Plasmid

#172213

Purpose

For allelic exchange in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.Lamp1-GFP

Plasmid

#180250

Purpose

Mammalian expression of LAMP1

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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