We narrowed to 9,122 results for: Pol

1. rat APOBEC1 E63A

   Plasmid

   #112861

   Purpose

   plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Expression

   Mammalian

   Mutation

   changed Glutamic acid 63 to Alanine

   Promoter

   CMV promoter

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a - CPX

   Plasmid

   #89620

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Cytosolic Tryparedoxin peroxidase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5 MYC-Smurf2 (FF29/30A) C716A

   Plasmid

   #24605

   Depositor

   Jeff Wrana

   Article

   Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

   Insert

   Smurf2 (SMURF2 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   F29A, F30A, C716A

   Available Since

   May 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pX330_SpCas9_USP7 Exon 3 gRNA

   Plasmid

   #131257

   Purpose

   pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   humanised S. pyogenes Cas9 nuclease
   

   Expression

   Mammalian

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB-­NIC-­II-­HisN-­TET12

   Plasmid

   #45496

   Depositor

   Roman Jerala

   Article

   Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

   Insert

   TET12
   

   Use

   Synthetic Biology

   Tags

   6xHistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMX155

   Plasmid

   #29481

   Depositor

   Linda Wordeman

   Article

   Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

   Insert

   Kif2A-alpha (Kif2a Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E140K

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLMB686

   Plasmid

   #83780

   Purpose

   myo-Inositol biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   upstream region of RL4655 (intA)
   

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET:GCAT:GFP

   Plasmid

   #60736

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGli3a

   Plasmid

   #25733

   Depositor

   C.C. Hui

   Article

   Hui et al Nat Genet. 1993 Mar . 3(3):241-6.

   Insert

   Gli3 (GLI3 Mouse)
   

   Expression

   Bacterial

   Available Since

   June 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCellFree_G03 PRKCA

    Plasmid

    #67115

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PRKCA Protein kinase C alpha type (PRKCA Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3.1-Muc2D3

    Plasmid

    #155216

    Purpose

    Expresses human Muc2 residues 858-1259

    Depositor

    Deborah Fass

    Article

    Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

    Insert

    Muc2 D3 (MUC2 Human)
    

    Tags

    6xHistadine

    Expression

    Mammalian

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a - SodB

    Plasmid

    #89613

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase B
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSicoR-mCh-Chd1i1

    Plasmid

    #21904

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    Chd1-i1
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET:ACAT:GFP

    Plasmid

    #60740

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. CS2-GFP-Ben

    Plasmid

    #47966

    Purpose

    Expression of fly Ben

    Depositor

    Laura Lee

    Article

    Merkle et al Development. 2009 Feb;136(3):449-59. doi

    Insert

    Bendless (ben Fly)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. CAG-EGFP-Otx2shRNA2

    Plasmid

    #73982

    Purpose

    Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    EGFP-Otx2-shRNA2
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-mCherry-CrxshRNA

    Plasmid

    #73987

    Purpose

    Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Crx gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AL13Rb-HsfA2

    Plasmid

    #161012

    Purpose

    PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HSP70-pp7i-mCh-UPG

    Plasmid

    #161011

    Purpose

    C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLMB684

    Plasmid

    #83779

    Purpose

    Xylose biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    upstream region of RL2720 (rbsC)
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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