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We narrowed to 15,275 results for: grn

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Showing: 4341 - 4360 of 15275 results

  5. tRNA-gRNA position [D1_2] (GB1205)

    Plasmid
    #75406
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75406-plasmid-map-sequence-id-146004

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  7. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid
    #73221
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL31-sgRNA NT1
    Expression
    Bacterial
    Promoter
    PL31
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73221-plasmid-map-sequence-id-135054

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  9. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  11. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid
    #234724
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
    Depositor
    Kirill Martemyanov
    Publication
    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234724-plasmid-map-sequence-id-466565

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  13. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

    Plasmid
    #210709
    Purpose
    This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Publication
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN (STAT1, MRTFA Synthetic, Human, Streptococcus pyogenes)
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210709-plasmid-map-sequence-id-416261

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Showing: 4341 - 4360 of 15275 results