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pet-MBP

Plasmid

#209184

Purpose

Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

Depositor

Attila Reményi

Publication

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Type

Empty backbone

Tags

HisX6

Expression

Bacterial

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-T7-kanMX6

Plasmid

#20760

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pET-His-Dre

Plasmid

#123130

Purpose

Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag.

Depositor

David Liu

Publication

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Dre recombinase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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wtBxb1_IVT

Plasmid

#222340

Purpose

Plasmid for wild type Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wtBxb1

Use

Template for in vitro transcription

Promoter

T7 (inactivated)

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBID-G

Plasmid

#35195

Depositor

Brian McCabe

Publication

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-LIMK2

Plasmid

#23834

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LIMK2 (LIMK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DCK

Plasmid

#23381

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCK (DCK Human)

Use

Gateway Cloning

Mutation

C57T

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pmCherry-C1-FAK-HA-V744G

Plasmid

#35040

Depositor

Anna Huttenlocher

Publication

Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

focal adhesion kinase (Ptk2 Mouse)

Tags

2x HA and mCherry

Expression

Mammalian

Mutation

GFP-FAK-V744G was generated from GFP-FAK using th…

Available since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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pDule-Tet3.0

Plasmid

#174080

Purpose

Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Publication

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

Tet3.0 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

L270G, Y271L, N311G, C313A

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-CSNK1G1

Plasmid

#23737

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1G1 (CSNK1G1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRSET B-CKAR

Plasmid

#14868

Depositor

Alexandra Newton

Publication

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

6xHis/Xpress, CFP, and YFP

Expression

Bacterial

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-CDK5

Plasmid

#23699

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK5 (CDK5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBSFlp

Plasmid

#45470

Depositor

Michele Swanson

Publication

Bryan et al Appl Environ Microbiol. 2011 Apr;77(7):2

Insert

Flp recombinase

Expression

Bacterial

Promoter

T7

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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TU#707

Plasmid

#15276

Depositor

Martin Chalfie

Publication

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKCZ

Plasmid

#23724

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCZ (PRKCZ Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CAMK1

Plasmid

#23544

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK1 (CAMK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-RIOK1

Plasmid

#23759

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIOK1 (RIOK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIP5K2C

Plasmid

#23450

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K2C (PIP4K2C Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-DAPK3

Plasmid

#23436

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DAPK3 (DAPK3 Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAS

Plasmid

#140008

Purpose

Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

4xPylT (Mma) cassette

Expression

Mammalian

Mutation

U25C

Promoter

h7SK

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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