We narrowed to 5,906 results for: pcDNA 3.1

1. JunD

   Plasmid

   #187904

   Purpose

   Expresses JunD in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunD (Jund Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NbAlfa-LgBit

   Plasmid

   #201483

   Purpose

   NanoBit detection of ALFA-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbALFA-LgBit
   

   Use

   Tags

   LgBit

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VinculinTS-A50I

   Plasmid

   #111827

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind talin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (A50I)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed alanine 50 to isoleucine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER2-mhYFP

   Plasmid

   #186528

   Purpose

   Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. DHFR-myc

   Plasmid

   #20214

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   DHFR (folA E. coli)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZZMP154

   Plasmid

   #231760

   Purpose

   Mammalian expression of LZ-SPO11

   Depositor

   Scott Keeney

   Article

   Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

   Insert

   LZ-SPO11 (Spo11 Budding Yeast, Mouse)
   

   Use

   Tags

   Flag-LeucineZipper

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VinculinTS-I997A

   Plasmid

   #111828

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed isoleucine 997 to alanine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH2.19_mCherry-MS2x2

   Plasmid

   #205534

   Purpose

   Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNIR-Fbactin

   Plasmid

   #184677

   Purpose

   NIR-Fb to actin

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fbactin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tTA_BFP

    Plasmid

    #58854

    Purpose

    Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    tTA transcription factor fused to enhanced blue fluorescent protein
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. myr-palm_mCherry2-mEGFP

    Plasmid

    #127816

    Purpose

    Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. rArc

    Plasmid

    #178206

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rArc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-CitroffRH

    Plasmid

    #158546

    Purpose

    Mammalian expression of nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM310-Myc

    Plasmid

    #80053

    Purpose

    Expression of a Myc tagged version of the BlaM-Vpr fusion protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-BlaM-Vpr
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal Myc tag added to construct pMM310

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cenp-B INCENP GFP

    Plasmid

    #45238

    Purpose

    Target INCENP to centromeres via full length CENP-B

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B, INCENP-Δcen, GFP
    

    Use

    Tags

    GFP and full length CENP-B

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ChlorON-3

    Plasmid

    #193479

    Purpose

    Turn-on fluorescent sensor for chloride

    Depositor

    Sheel Dodani

    Article

    Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

    Insert

    ChlorON-3
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZ148-BhCas12b-sgRNA-scaffold

    Plasmid

    #122448

    Purpose

    Expresses sgRNA for BhCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BhCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RNT2-GFP

    Plasmid

    #17709

    Depositor

    Hal Dietz

    Article

    Mendell et al Mol Cell Biol. 2000 Dec . 20(23):8944-57

    Insert

    rent2 (UPF2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pVH233-Tier1-PmPGK1-eYFP

    Plasmid

    #169522

    Purpose

    Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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