We narrowed to 5,034 results for: PID

1. pJFRC7-JEDI-2P

   Plasmid

   #179461

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNK116

   Plasmid

   #219742

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-DNER

   Plasmid

   #51053

   Purpose

   expression of HA-DNER in mammalian cells

   Depositor

   Mineko Kengaku

   Article

   Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

   Insert

   DNER (Dner Mouse)
   

   Use

   Tags

   HA and signal peptide

   Expression

   Mammalian

   Mutation

   without endogenous signal peptide (1-221bp)

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. JWW-1 human chimeric antibody

   Plasmid

   #66748

   Purpose

   Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

   Depositor

   Richard Roden

   Article

   Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

   Inserts

   JWW-1 Heavy Chain Gamma
   JWW-1 Light Chain Kappa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   First 3 amino acids of rat light chain constant r…

   Promoter

   mEF1 and rEF1

   Available since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22-His-TEV-DOT1L

   Plasmid

   #89610

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

   Plasmid

   #170537

   Purpose

   Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 151-175

   Promoter

   CAG promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pETM6-4F2-mCherry

   Plasmid

   #73421

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 4F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P4F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. ReactionC_pBIG1a_ATG7_ATG10

   Plasmid

   #190860

   Purpose

   Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG7 (ATG7 Human)
   ATG10 (ATG10 )
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-TagRFP658-P2A-GFP

    Plasmid

    #178971

    Purpose

    Expresses TagRFP658 and GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag_HsIRE1a_deltaP29_D408_pBabePuro

    Plasmid

    #58422

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    Deleted amino acids P29 to D408

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. LZRS-BRaf-ERTm

    Plasmid

    #24949

    Depositor

    Paul Khavari

    Article

    Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

    Insert

    BRaf-ER (Esr1, BRAF Mouse, Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    V600E in BRAF

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pExp-RBD-CHis

    Plasmid

    #195002

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ps-3HA-Beta2-PSCFP2-N

    Plasmid

    #114186

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA and PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86022

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain; Changed alanine 82 to va…

    Promoter

    CMV IE

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. VBS mutant of alpha catenin sensor

    Plasmid

    #71710

    Purpose

    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin ΔVBS FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 316-405 in α -catenin…

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-Sec22b-P33-shR

    Plasmid

    #208359

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations (shRNA resistance), 33 aa p…

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEL1200

    Plasmid

    #138526

    Purpose

    For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

    Insert

    mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)
    

    Use

    Tags

    N-terminal His tag and TEV cleavage site on MlaD

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Hyg-mEGFP-pp4640

    Plasmid

    #191846

    Purpose

    Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP-pp4640
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    mEGFP fusion in Nterminal, some silent point muta…

    Promoter

    CMV

    Available since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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