We narrowed to 18,681 results for: tec

1. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

   Plasmid

   #138152

   Purpose

   Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   eGFP-MCS
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY1243-Fluorescent RADARS

   Plasmid

   #182540

   Purpose

   Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   mNeon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 ZoAl-ENT N-1F (R1103A)

   Plasmid

   #216548

   Purpose

   For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PrimeRoot.v2C-Cre

   Plasmid

   #201739

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSplit2-NO-PRPF6

   Plasmid

   #51740

   Purpose

   Express LucN-PRPF6 fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   PRP6 (PRPF6 Human)
   

   Use

   Tags

   FLAG and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH-AFID-3

   Plasmid

   #167361

   Purpose

   For inducing predictable deletions in rice plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCoofy57

   Plasmid

   #122009

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His8 and Multi1: depending on the LP2 primer, eit…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTF-ahpC-rfp

   Plasmid

   #153035

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TCARZ-CBh-GFP-SV40PA-GF3-R4A22

    Plasmid

    #203767

    Purpose

    For production of template RNA for use in PRINT, GFP under CBh promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid

    #167583

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SaABE8e

    Plasmid

    #138500

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTlpA_mCherry_Txe/Axe

    Plasmid

    #229147

    Purpose

    Toxin-Antitoxin (Txe/Axe)

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry, Txe/Axe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtlpA (mCherry), Txe/Axe (Native)

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCpf1b-sp

    Plasmid

    #122188

    Purpose

    Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYL-kasOp-FnCas12a3

    Plasmid

    #177875

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized the FnCas12a mutant EP16
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2CT-His-MBP-Lbu_C2c2_WT

    Plasmid

    #83482

    Purpose

    Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMB1-spect-PthrC3_8-eGFP

    Plasmid

    #107411

    Purpose

    This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC3_8

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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