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Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only
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pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD-MSP1D1
Plasmid#161607PurposeBacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formationDepositorInsertD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans were replaced b…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS SK mCherryROSAegfp
Plasmid#54322PurposeEncodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.DepositorInsertmCherry and EGFP
UseCRISPR, Mouse Targeting, and TALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-mRFP1
Plasmid#53745PurposeContains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsmRFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0532
Plasmid#133502PurposeExpression of mTagBFP2 targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0526
Plasmid#133500PurposeExpression of sfGFP targeted to the nucleus with one NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-EGFP
Plasmid#53744PurposeContains human Histone H2B fused to EGFP in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsEGFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJune 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0761
Plasmid#133494PurposeExpression of nucleo-cytoplasmic mTagBFP2 in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only